结外NK/T细胞淋巴瘤（鼻型）microRNA的表达研究

Expression of microRNA in extranodal NK/T cell lymphoma, nasal type

目的 通过分析结外NK/T细胞淋巴瘤（鼻型）肿瘤组织中microRNA（ miRNA）的表达情况，探索其分子改变特征。方法 对1例结外NK/T细胞淋巴瘤（鼻型）和1例炎性鼻黏膜组织应用TaqMan低密度芯片分析665种miRNA的表达差异，发现95种miRNA存在变化。结合文献复习和数据库检索，从其中筛选差异明显、重要的8种miRNA。应用即时定量聚合酶链反应方法进一步对15例结外NK/T细胞淋巴瘤（鼻型）与3例炎性鼻黏膜组织验证这8种miRNA的表达情况。结果 8种miRNA验证结果与表达谱结果对比表达趋势一致，经统计学处理，其中3种重要miRNA[miR-223和miR-886-3p在结外NK/T细胞淋巴瘤（鼻型）组织中表达上调，miR-34c-5p表达下调]表达差异有统计学意义（P值分别为0.002、0.010和0.017）。结论 造血系分化发育、细胞增殖凋亡有关的miRNA：miR-223、886-3p和34c-5p在结外NK/T细胞淋巴瘤（鼻型）表达有明显异常，其是否参与肿瘤分子遗传学的改变及其意义值得深入探讨。

Objective To study the expression of microRNAs （miRNAs） in extranodal NK/T cell lymphoma, nasal type （EN-NK/T-NT）. Methods TaqMan low density arrays were used to assess the expression level of 665 miRNAs in one case of EN-NK/T-NT and one normal nasopharyngeal tissue. Ninetyfive miRNAs were found to be aberrantly expressed （86 being up-regulated and 9 being down-regulated） in EN-NK/T-NT, compared with normal nasopharyngeal tissue. The aberrant expression was found to be most significant in 8 miRNAs. According to the literature and miRNA database, the expression patterns of these 8 miRNAs were further analyzed in 15 cases of EN-NK/T-NT and 3 normal nasopharyngeal specimens, using the single tube TaqMan microRNA assays. Results Three of the 8 miRNAs showed statistically significant difference in the expression in EN-NK/T-NT and normal nasopharyngeal specimens. These 3 miRNAs （ miR-223, 886-3p and 34c-5p） were considered to play crucial roles in hemopoiesis, cellular proliferation and apoptosis. MiR-223 and miR-886-3p were significantly over-expressed in EN-NK/T-NT （ P =0. 002, P =0. 010） while miR-34c-5p was significantly under-expressed （P =0. 017）. Conclusions Certain types of miRNAs, especially those related to hemopoiesis, cellular proliferation and apoptosis, are aberrantly expressed in EN-NK/T-NT. The potential role of miRNAs in the molecular genetics of EN-NK/T-NT requires further investigation.