靶向STAT3的RNA干扰对人胃癌细胞增殖和侵袭的影响

Effects of RNAi targeting STAT3 on the proliferation and invasion of human gastric carcinoma cell

目的观察瞬时转染信号转导与转录激活因子3(STAT3)siRNA后对人胃癌细胞SGC-7901增殖、凋亡与侵袭的影响。方法以人胃癌细胞系SGC-7901培养瞬时转染特异性siRNA的SGC-7901细胞系,应用RT-PCR、MTT、流式细胞术和Transwell体外侵袭实验等检测该siRNA对SGC-7901细胞基因表达、细胞增殖、细胞周期、细胞凋亡及侵袭转移能力的影响。结果 STAT3siRNA转染SGC-7901细胞48 h后,STAT3 mRNA水平下降了73.04%,细胞增殖受到明显抑制,抑制率45.73%;STAT3 siRNA组G0-G1期细胞比例增加21.03%,S期细胞比例减少15.24%,细胞凋亡率升高了6.97%;STAT3 siRNA组细胞侵袭力下降了45.26%(P<0.01)。结论应用siRNA技术能有效抑制SGC-7901 STAT3基因的表达,进而抑制细胞的增殖,诱导细胞凋亡,降低其侵袭力,为以STAT3为靶向的胃癌基因治疗提供了新的思路和手段。

Objective To study the effects of RNAi targeting STAT3 transient transfection on the proliferation,apoptosis and invasion of human gastric carcinoma cell SGC-7901.Methods SGC-7901 was cultured and transiently transfected by specific siRNA.RT-PCR was used to mesure the level of STAT3 mRNA in the cells,MTT method was used to detect the proliferation of the cells and the cell growth curve was depicted,cell cycle and apoptosis were examined via flow cytometry,and the invasiveness of SGC-7901 cells in vitro was measured quantitatively by the invasion test of Transwell chamber.Results Compared with two control groups,after 48 hours,the level of STAT3 mRNA decreased by 73.04%,the proliferation was obviously inhibited by 45.73%,the proportion of G0-G1 stage cells increased by 21.03%,S stage cells decreased by 15.24%,and cell apoptosis increased by 6.97%.Transwell invasion in vitro was also reduced by 45.26%（P0.01）.Conclusion Silence STAT3 by siRNA technology can effectively inhibit the expression of STAT3 mRNA,lead to supressing the proliferation of human gastric carcinoma,inducing cell apoptosis,degrading the invasiveness of gastric carcinoma.It may provide a new clue in targeting therapy of human gastric carcinoma.