反义ATM在体外对喉鳞状细胞癌ATM蛋白表达影响的研究

Study on the Protein Expression of Laryngeal Squamous Cell Carcinoma ATM by ATM Antisense Oligodeoxynucleotides in Vitro

目的:用ATM反义多核苷酸作用于喉鳞状细胞癌以研究其对ATM蛋白表达的影响,进而推测其对放射治疗的敏感性。方法:用Western blot对AS、Sen、Misl、ipo与hep-2组的ATM蛋白进行分析。结果:AS、Sen、Misl、ipo与hep-2组(对照组)的ATM蛋白相对表达量以AS-lipo组最低,为48.14±5.53%,AS组与Sen、Misl、ipo、hep-2组比较有显著性差异。结论:ATM反义多核苷酸降低了喉鳞状细胞癌细胞中ATM蛋白的表达,其可能使喉鳞状细胞癌细胞对放射治疗的敏感性增强。

Objective：We used antisense oligodeoxynucleotides（AS-ODNs） to hep2（Human epidermoid laryngeal carcinoma） cells to study protein expression of ATM,and to analyze the sensitivity of hep-2 cells to radiation in vitro.Methods： In groups of AS、Sen、Mis、lipo and hep-2,the expression of ATM protein were examined by western blotting respectively.Results： The relative ATM protein expression in hep-2 cells treated with ATM AS-ODNs were decreased to 48.14±5.53% of that in untreated cells respectively（p0.05）.There are no significant differences among liposome-treated group（Lipo）,Sen-ODNs（Sen-Lipo） treated group and Mis-ODNs（Mis-Lipo） treated group（p0.05）,while the group treated with ATM AS-ODNs notably different compared with other groups（p0.05）.Conclusion： AS-ODNs of ATM canreduce ATM expression and may enhance hep-2 cells sensitive to radiation in vitro.