基于高分辨率熔解曲线技术快速筛选黄瓜SNP_1

Discovery of Single Nucleotide Polymorphism in Cucumber Based on High Resolution Melting Analysis

为筛选用以黄瓜纯度检验和品种鉴定的SNP位点,以17个黄瓜杂交品种及其34份亲本为SNP筛选群体,利用高分辨率熔解曲线(high resolution melting,HRM)技术筛选SNP位点。本研究通过HRM分析和焦磷酸测序确认,获得CLA0(TT/--)、CLA1(C/T)和CLA6(A/G)三个SNP位点,群体中多态信息量(PIC)分别为0.549、0.366和0.477;并对市售12个杂交黄瓜种分型鉴定,结果表明,三个SNP位点适用于其中11个市售品种的纯度鉴定,具备用于品种鉴定的潜能。

In order to explore SNP sites for cucumber purity test and cultivar identification, high resolution melting （HRM） was used to screening SNP site from the group including 17 cucumber hybrid and their parents lines. By HRM analysis and Pyrosequencing confirmation, we got three SNP sites, named CLA0（TT /--）, CLA1（C/T） and CLA6（A/G）, whose polymorphism information （PIC） were 0.549, 0.366 and 0.477 respectively. And the three SNP sites are suited to purity test for 91.7% of the 12 commercial cucumber hybrid cultivars and have the potentiality for cultivar identification.