摘要
目的:采用全细胞膜片钳记录技术研究Sombati癫癎细胞模型电压依赖性K+电流的变化。方法:取SD新生乳鼠双侧海马,体外原代培养海马神经元,培养至第9天的部分海马神经元经无镁液处理3h制备癫癎细胞模型,采用全细胞膜片钳技术分别记录正常组(未经无镁液处理的海马神经元)和致癎组(经无镁液处理3h后更换为正常维持培养液的海马神经元)海马神经元的电压依赖性外向K+电流。结果:致癎组外向K+电流比正常组增大,峰值电流由(596.62±79.23)pA增至(978.68±53.23)pA,两组间比较差异有统计学意义(P=0.000)。与正常组相比,致癎组的I-V曲线明显左移,I-V曲线形态无显著改变。结论:Sombati癫癎细胞模型中,电压依赖性外向K+电流显著增加可能是癫癎细胞模型癎性放电后为了保持细胞稳态的代偿性保护作用。
Aim：To study the changes of voltage-dependent K＋ current in Sombati cell model of epilepsy by using whole-cell patch clamp technique.Methods：The hippocampal tissues were sampled from 1-day-old SD rats and used for the primary culture of hippocampus neurons in vitro.After 9 days of primary culture,the hippocampal neurons were randomly divided into two groups：the normal group and the epilepsy group,which voltage-dependent K＋ current were recorded by whole cell patch clamp technique respectively.Results：The K＋ currents in the epilepsy group increased significantly than that in the normal group,and the peak current in the epilepsy group increased from（596.62±79.23） pA to（596.62±79.23） pA（P=0.000）.The differences were significant statistically between the two groups.Compared with the normal group,I-V curve of the epilepsy group was shifted to the left obviously,but the shape of the curve remained unchanged.Conclusion：The voltage-dependent outward K＋ current in Sombati cell model of epilepsy increased significantly,which may be the result of maintaining compensatory protective effect on cell homeostasis after discharge.
出处
《中国临床神经科学》
2011年第5期449-453,共5页
Chinese Journal of Clinical Neurosciences
基金
国家自然科学基金课题(编号:30960111)
广西科技厅面上项目(编号:2010GXNSFA013168)
