草苁蓉环烯醚萜苷对兔VX2移植瘤细胞增殖和凋亡的作用

Effects of iridoid glucosides from the Boschniakia rossica on proliferation and apoptosis of transplanted with VX2 tumor cells in rabbits

[目的]探讨草苁蓉环烯醚萜苷(IGBR)对兔VX2移植瘤细胞增殖和凋亡的影响.[方法]将VX2移植瘤兔分为模型对照组、氟尿嘧啶(5-Fu)组和IGBR小、大剂量组.于建立模型第4d开始给药,小、大剂量IGBR组按照每日50,100mg/kg的剂量连续灌胃给药21d,5-Fu组按照每日25mg/kg的剂量隔日腹腔注射给药3次.实验末期,提取瘤体标本进行病理学观察,采用TUNEL法观察肿瘤细胞凋亡,免疫组织化学法检测移植瘤细胞Bcl-2,Bax及增殖细胞核抗原(PCNA)的表达情况.[结果]IGBR可显著抑制肿瘤细胞PCNA表达,诱导肿瘤细胞凋亡,并降低Bcl-2蛋白表达和增高Bax蛋白表达.[结论]抑制肿瘤细胞增殖和诱导肿瘤细胞凋亡可能是IGBR抑制肿瘤生长作用机制之一.

OBJECTIVE To study the effects of iridoid glucosides from the Boschniakia rossica （IGBR） on proliferation and apoptosis of transplanted with VX2 tumor cells in rabbits. METHODS Rabbits transplanted with VX2 tumor cells were randomly divided into 4 groups, including groups of control, 5-Fu, low and high dose of IGBR. Animals were treated with drugs after establishing models for 4 days. The rabbits in groups low and high dose of IGBR were administrated with 50 and 100 mg/kg per day IGBR i.g. once daily for 21 days, and in group of 5-Fu was treated with 5-Fu 25 mg/kg i.p. every other day for 3 times. At the end of experiment, excised tumor samples were observed by pathology, and the cell apoptosis was analyzed with TUNEL, and the expressions of Bcl-2, Bax and proliferation cell nuclear antigen （PCNA） in transplanted VX2 tumor cells were determined by immunohistochemistry. RESULTS The administration with IGBR reduced the expression of PCNA of VX2 tumor cells, and induced apoptosis of tumor cells, decreased the expression of Bcl-2 and increased the expression of Bax protein. CONCLUSION The anti-tumor mechanism of IGBR maybe related to the inhibition of cell proliferation and the induction of cell apoptosis.