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pheS基因序列分析在干酪乳杆菌群种水平鉴定中的应用 被引量：9

Species Identification of the Lactobacillus casei Group by pheS Gene Sequences Analysis
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摘要 对干酪乳杆菌群(Lactobacillus casei group)内5个种和4个亚种的23个代表菌株的苯丙氨酰-tRNA合成酶α亚基基因(pheS)序列和18个16S rRNA基因序列进行系统发育学分析,探索pheS基因序列分析在干酪乳杆菌群种水平鉴定中应用的可行性。分析结果表明,pheS基因序列群内种间的差异率在6.8%～34%之间,种内最大差异率可达3%,其变异率远高于16S rRNA基因,可作为该群内种水平鉴定的重要手段。 The phenylalanyl-tRNA synthase alpha subunit （pheS） gene sequences and 16S rRNA gene sequences of representative strains of 5 species and 4 subspecies from Lactobacillus casei group were analyzed by phylogenic method. The feasibility of the pheS gene sequences for species identification of the Lactobacillus casei group was studied. Results showed that the interspecies divergence of pheS gene sequences was between 6.8% N 34% and the highest intra-species divergence could reach 3%. The discriminatory power ofpheS gene sequences was higher than that of 16S rRNA gene sequences, the pheS gene sequences analysis could be an important tool in species identification in the LactobaciUus casei group.
作者 刘光全 刘勇 李辉 程池
机构地区 中国食品发酵工业研究院
出处 《食品与发酵工业》 CAS CSCD 北大核心 2011年第9期94-97,共4页 Food and Fermentation Industries
基金 国家十一五科技支撑计划课题(2007DKA36B06)
关键词 pheS基因 干酪乳杆菌群 菌种鉴定 pheS gene, Lactobacillus casei group, species identification
分类号 TS201.3 [轻工技术与工程—食品科学]
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参考文献18

  • 1马凯,李春玲,程池.Lactobacillus的系统发育分析及分群[J].食品与发酵工业,2007,33(2):27-35. 被引量：6
  • 2Naser S M,Thompson F L,Hoste B,et al.Application ofmultilocus sequence analysis (MLSA)for rapid identifi-cation of Enterococcus species based on rpoA and pheSgenes. Microbiology . 2005
  • 3Naser S M,Dawyndt P S R,Hoste B Gevers,et al.I-dentification of lactobacilli by pheS and rpoA gene se-quence analyses. International Journal of Systematic and Evolutionary Microbiology . 2007
  • 4DSMZ (Deutsche Sammlung von Mikroorganismen undZellkulturen GmbH Braunschweig,Germany).Bacte-rial Nomenclature Up-to-date (Approved lists,validationlists). . 2011
  • 5The type strain of Lactobacillus casei is ATCC 393,ATCC334 cannot serve as the type because it represents a differ-ent taxon,the name Lactobacillus paracasei and its sub-species names are not rejected and the revival of the name’’Lactobacillus zeae’’contr. International Journal of Systematic and Ev-olutionary Microbiology . 2008
  • 6Dicks L M T,Du P,Dellaglio F,et al.Reclassification ofLactobacillus casei subsp.casei ATCC 393 and Lactobacillusrhamnosus ATCC 15820 as Lactobacillus zeae nom.rev.designation of ATCC 334 as the neotype of L.casei,and re-jection of the name Lactobacillus paracasei. Int J SystBacteriol . 1996
  • 7Zhong W,Millsap K,Bialkowska-Hobrzanska H,et al.Differentiation of Lactobacillus species by molecular typing. Applied and Environmental Microbiology .
  • 8Chen H,Lim C K,Lee Y K,et al.Comparative analysis ofthe genus encoding 23S-5S rRNA intergenic spacer re-gions of Lactobacillus casei-related strains. Int J SystBacteriol . 2000
  • 9Felis G E,Dellaglio F,Mizzi L,et al.Comparative se-quence analysis of recA genes fragment brings new evi-dence for a renewal in the taxonomy of the Lactobacilluscasei group. International Journal of Systematic and Evolutionary Microbiology . 2001
  • 10Mori,K. et al.Comparative sequence analyses of the genes coding for 16S rRNA of Lactobacillus casei-related taxa. International Journal of Systematic Bacteriology . 1997

二级参考文献30

  • 1DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH Braunschweig,Germany).Bacterial Nomenclature Up-to-date (Approved lists,validation lists),2006
  • 2Mori K,Yamazaki K.Comparative Sequence Analyses of the Genes Coding for 16S rRNA of Lactobacillus caseiRelated Taxa[J].Int J Syst Bacteriol,1997,47:54～57
  • 3Mukai T,Arihara K,Ikeda A,et al.Lactobacillus kitasatonis sp.Nov.,from chicken intestine[J].Int J Syst Evol Microbiol,2003,53:2 055～2 059
  • 4Dicks L M T,Du Plessis E M,Dellaglio F,et al.Reclassification of Lactobacillus casei subsp.casei ATCC 393 and Lactobacillus rhamnosus ATCC 15820 as Lactobacillus zeae nom.rev.,designation of ATCC 334 as the neotype of L.casei subsp.casei,and rejection of the name Lactobacillus paracasei[J].Int J Syst Bacteriol,1996,46:337-340
  • 5Dellaglio F,Giovanna E,Felis,et al.The status of the species Lactobacillus casei (Orla-Jensen 1916) Hansen and Lessel 1971 and Lactobacillus paracasei Collins et al.1989.Request for an Opinion[J].Int J Syst Evol Microbiol,2002,52:285～287
  • 6Thompson J D,Higgins D G,Gibson T J:CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,positions-specific gap penalties and weight matrix choice[J].Nucleic Acids Research,1994,22:4 673～4 680
  • 7Sudhir K,Koichiro T,Ingrid B J,et al.MEGA2:Molecular Evolutionary Genetics Analysis software,Arizona State University,Tempe,Arizona,USA.2001
  • 8Kostinek M,Pukall R,Rooney A P,et al.Lactobacillus arizonensis is a later heterotypic synonym of Lactobacillus plantarum[J].Int J Syst Evol Microbiol,2005,55:2 485～2 489
  • 9Collins M D,Rodrigues U,Ash C,et al.Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA[J].FEMS Microbiology Letters,1991,77:5
  • 10Pettersson B,Priest F G,Kirsten L,et al.Characterization of lactobacilli from Scotch malt whisky distilleries and description of Lactobacillus ferintoshensis sp.nov.,a new species isolated from malt whisky fermentations[J].Microbiology,2001,147:1 007～1 016

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食品与发酵工业
2011年 第9期

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