罗非鱼鱼皮鱼鳞蛋白的酶解及超滤分离

Characteristics of Enzymatic Hydrolysates of Tilapia Skin Protein and Effects of Ultrafiltration on It

采用Alcalase2.4L、Protamex、Papain、PTN6.0S和Neutrase酶解罗非鱼鱼皮鱼鳞蛋白,探讨了超滤对酶解液分子质量分布和氨基酸组成的影响。结果表明,Alcalase2.4L、PTN6.0S酶解产物的蛋白质回收率、肽得率和水解度均较高,Alcalase2.4L∶PTN6.0S为1∶2添加时酶解效果最好,蛋白回收率为96.37%,水解度为13.24%,肽得率为83.13%。经超滤处理后,3000 u以下分子质量的肽段达到97.73%,比超滤前增加了5.37%,总氨基酸含量由84076.12 mg/100 g增加到97234.79 mg/100 g。

Tilapia skin protein was hydrolyzed by Alcalase2.4L, Protamex, Papain,PTN6.0S, Neutrase enzymes. The hydrolysis reactions catalyzed by these enzymes were studied. Results revealed that the protein utilization, peptide yield and the degree of hydrolysis of Tilapia skin protein by PTN6.0S and Alcalase2.4L were higher than those by the other enzymes. Among the achieved hydrolysates, the hydrolysate achieved with Alcalase2.4L： PTN6.0S（1：2） had the best result,the protein utilization, the degree of hydrolysis and peptide yield were respectively 96.37% , 13.24% and 83.13%. After the uhrafihration treatment, the ratio of the molecular weight less than 3000 u reached 97.73% , the total amino acid content increased from 97234.79mg/100g to 84076. 12mg/100g.