马铃薯块茎粗多酚体外抗氧化活性

Antioxidant Activities of Crude Polyphenol Extracts from Potato Tuber in vitro

对马铃薯块茎粗多酚抗氧化活性进行研究。采用乙醇、丙酮、蒸馏水3种溶剂提取马铃薯块茎中粗多酚类物质,通过粗多酚提取液对羟自由基(.OH)、超氧自由基(O2-.)、亚硝酸盐(NO2-)的清除和还原能力测定,比较3种酚提液的体外抗氧化活性,并测定其中总多酚含量。结果显示:3种粗多酚提取液总多酚含量为0.33～0.40 mg/mL;在不同抗氧化体系中,3种酚提液均具有良好的抗氧化活性,抗氧化能力的大小顺序为60%乙醇酚提液>60%丙酮酚提液>蒸馏水酚提液,抗氧化活性的大小与总多酚含量的高低呈正相关。

The antioxidant activities of crude polyphenol extracts from potato tuber were studied. The crude poly- phenol extracts were extracted using ethanol, acetone and water. Antioxidant activities of three polyphenol extracts were studied including ＇ OH free radical scavenging , O2-· free radical scavenging , NO2- scavenging and reducing power. The contents of polyphenol were also determined. Results showed that total polyphenol contents were 0.33 0.40 mg/mL. Three crude polyphenol extracts exhibit excellent antioxidation activitiy in different antioxidant assays. The order of antioxidation abilities were ： 60% ethanol extract 〉 60% acetone extract 〉 water extract. This was positively correlated with the total polyphenol contents of the different polyohenol extracts.