板蓝根的化学成分研究

Chemical constituents of in roots of Isatis indigotica

目的研究板蓝根乙醇提取物的醋酸乙酯和正丁醇萃取层中的化学成分。方法采用反复硅胶柱色谱、凝胶柱色谱法进行分离纯化,根据化合物的理化性质和波谱数据鉴定化合物的结构。结果分离鉴定了12个化合物,分别为喜果苷(1)、quinovic acid-3β-O-β-D-glucopyranosyl-(28→1)-β-D-glucopyranosyl ester(2)、quinovic acid-3β-O-β-D-glucopyranosyl(3)、棕榈酸甲酯(4)、水杨酸(5)、远志醇(6)、腺苷(7)、靛玉红(8)、靛蓝(9)、大黄素(10)、β-谷甾醇(11)和β-胡萝卜苷(12)。结论化合物1、2、3为首次从十字花科植物中分离得到,化合物4为首次从菘蓝属植物中分离得到。关键词:板蓝根;十字花科;菘蓝属;喜果苷;棕榈酸甲酯;

Objective To study the chemical constituents in acetic ether and n-butyl alcohol fractions from the roots of Isatis indigotica alcohol extract. Methods The compounds were isolated by a combination of silica gel column and ODS column chromatography. Physicochemical characteristics and spectroscopic evidences were employed for the structural identification. Results Twelve compounds were isolated and identified as vincoside-lactam （1）, quinovic acid-3β-O-β-D-glucopyranosyl-（28→1）-β-D- glucopyranosyl ester （2）, quinovic acid-313-O-β-D-glucopyranoside （3）, methyl palmitate （4）, salicylic acid （5）, polygalitol （6）, adenosine （7）, indirubin （8）, indigotin （9）, emodin （10）, 13-sitosterol （11）, and β-daucosterol （12）. Conclusion Compounds 1, 2, and 3 are identified for the first time from the plants in family Cruciferae, compound 4 is identified for the first time in the plants of Isatis L.