超高效液相色谱法测定安乐片中甘草苷和2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量

UPLC determination of liquiritin and 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-gulcoside in Anle Pian

目的用UPLC法测定安乐片中甘草苷和2,3,5,4-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量。方法色谱柱为Waters BEHC18（50 mm×2.1 mm×1.7μm）,流动相为乙腈-水（10∶90）,流速为0.2 mL.min-1,检测波长为280 nm,柱温20℃。结果甘草苷在133.25-2 665 ng与峰面积线性关系良好（r=0.999 8）,2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在127.25-2 545 ng与峰面积线性关系良好（r=0.9997）,其平均回收率（n=6）分别为99.3%（RSD为1.6%）和96.6%（RSD为1.7%）。结论本法灵敏、准确、专属性强,能简便有效的控制安乐片中甘草苷和2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量。

Objective To establish a UPLC method for the determination of liquiritin and 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-gulcoside in Anle Pian.Methods The chromatographic procedure acetonitrile-water（10∶90） was carried out on a Waters BEH C18（50 mm×2.1 mm×1.7 μm） column.The mobile phase was acetonitrile-water（10∶90） at 0.2 mL·min-1 and the detective wavelength was 280 nm.The column temperature was 20 ℃.Results The linear range of liquiritin was 133.25-2 665 ng（r=0.999 8）,while that of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-gulcoside was 127.25-2 545 ng（r=0.999 7）.The average recovery（n=6） was 99.3%（RSD=1.6%） and 96.6%（RSD=1.7%）.Conclusion The method is highly sensitive,reliable,and accurate,which can be applied in the quality control of Anle Pian.