摘要
以啤酒糟为主要原料,研究了黑曲霉A-1固态发酵生产富酶蛋白饲料的培养基及工艺条件。试验结果表明:最适发酵培养基为啤酒糟:棉粕比例为5:5,KH2PO40.2%,最适发酵条件为:料水比为1:1.0,接种量为含4.0×106个孢子的孢子悬液,发酵温度为30℃,培养时间为54h～60h,纤维素酶达到1991u/g,木聚糖酶达到1998u/g,酸性蛋白酶达到4559u/g,粗蛋白为37.4%。
For Protein-rich feed production by Aspergillus niger A-1 using brewer＇s grains as the main material,solid-state fermentation conditions was studied and optimized in this paper.The results showed that the optimal condition was as follows：the ratio of brewer＇s grains and mianpo in medium 5：5,the content of KH2PO4 0.2%;the best fermentation conditions were that the drier-water ratio was 1：1.0,the inoculum of 4.0×106 spores in spore suspension,the temperature 30℃,the time for fermentation 54h～60h.Under those conditions,the enzyme activity of cellulase produced by the strain A-1 reached 1991u/g,xylanase enzyme activity 1998u/g,the activity of acid proteinase 4559u/g and the crude protein 37.4%.
关键词
黑曲霉
啤酒糟
固态发酵
纤维素酶
酸性蛋白酶
木聚糖酶
粗蛋白
Aspergillus niger
brewer＇s grains
solid-state fermentation
cellulase
acid proteinase
xylanase
crude protein
