肝细胞生长因子激活物抑制因子-1基因在肾癌组织中的表达及其与肿瘤血管生成的关系

Expression of helmtocyte growth factor activator inhibitor-1 gene in the tissues of renal cell earcinoraa and the correlation with tumor angiogenesis

目的探讨肝细胞生长因子激活物抑制因子-I（HAI-1）基因在肾癌组织中的表达及其与肿瘤血管生成的关系。方法应用半定量反转录．聚合酶链反应（RT-PCR）法检测HAI-1基因在56例肾癌组织和相应癌旁非癌肾组织及12倒正常肾组织中的表达水平；采用免疫组织化学SP法测定上述组织中CD34的表达，并测定微直管密度（醚VD），分析HAI-1基因的表达与肾癌临床瘸理特征及MVD的关系。结果HAI-1基因在肾癌组织、癌旁非癌肾组织、正常肾组织中的阳性率分别为58．93％（33/56）、76．79％（43156）和91．67％（11／12），肾癌组织中HAI-1基因阳性率显著低于癌旁非癌’纾组织和正常肾组织（P〈0．05）；HAI-1基因的表达与Fuhrman病理分级、Robson临床分期呈负相关（r=-0．617、-0．432，P〈0．01或〈0．05）；肾癌组织有淋巴结转移者的HAI-1基因阳性率[35．29％（6／17）]显著低于无淋巴结转移者[69。23％（27/39）]，差异有统计学意义（P〈0．05）。肾癌组织中MVD显著高于癌旁非癌肾组织和正常肾组织，差异有统计学意义（P〈0．05）；肾癌组织中HAI-1基因的表达与MVD呈负相关（r=-0．516，P〈0．01）。结论HAI-1基因表达缺失与肾癌的发生和发展、浸润和转移存在一定的关系，HAI-1基因表达缺失可作为肾癌浸润、转移和预后的一种重要分子标志。

Objective To investigate the expression of hepatocyte growth factor activator inhibitor- 1 （HAI-1） gene in the tissues of renal cell carcinoma （RCC） and the correlation with tumor angiogenesis. Methods The expression of HAI-1 gene was detected by semi-quantitative RT-PCR in the tissues of 56 cases of RCC, corresponding adjacent non-cancer tissues and 12 normal renal tissues, the expression of CD34 was detected by immunohistochemistry SP method in the above-mentioned organizations, and counted the microvessel density （MVD）,analyzed the relationship between the expression of HAI-1 gene in RCC and clinicopathological parameters, MVD. Results The positive expression rate of HAI-1 gene in RCC tissues, the adjacent non-cancer tissues and normal renal tissues were 58.93%（ 33/56 ）, 76.79%（ 43/56 ） and 9 1.67% （ 1 i[12）, respectively. The expression of HAI-1 gene was a negative correlation to the Fuhrman histological grade, Robson clinical stage（r = -0.617 ,-0.432,P 〈 0.01 or 〈 0.05 ）. The positive expression rate in lymph node metastasis was significantly lower than that in without lymph node metastasis [ 35.29% （6/17） vs. 69.23%（27/39） ,P 〈 0.05 ]. The MVD in RCC tissues was significantly higher than that in the adjacent non-cancer tissues and normal renal tissues（P 〈 0.05 ）. The expression of HAI-1 gene in RCC tissues was negative correlated to the MVD （r =-0.516,P 〈 0.01 ）. Conclusions There is a certain relationship between the missing expression of HAI-1 gene in RCC and the development,invasion and metastasis of RCC. The HAI-1 gene deletion can be used as an important molecular marker in the invasion and metastasis of RCC.