摘要
目的研究分离山楂中熊果酸与齐墩果酸的最佳工艺,建立RP-HPLC法测定山楂药材中熊果酸的方法。方法采用高效液相色谱法,C18色谱柱(250 mm×4.6 mm×5μm),流动相为甲醇—水—冰乙酸(83∶17∶0.04);柱温:25℃;流速:1.0 ml/min;检测波长:220 nm。结果熊果酸进样量在0.071 4～4.284 mg/ml范围内与峰面积成良好的线性关系,r=0.999 7,平均回收率为101.66%,RSD=1.70%。结论该方法精密度好,结果准确可靠。
[Objective] To find out the optimized process for ursolic acid and oleanolic acid in hawthorn,and establish the method for determination of ursolic acid in hawthorn herbs by RP-HPLC. [Methods]RP-HPLC method was adopted,with column C 18（250 mm×4.6 mm×5 μm）,methanol-water-acetic acid as mobile phase（83∶17∶0.04）,Column temperature： 25 ℃,flow rate： 1.0 ml / min,detection wavelength： 220 nm. [Results]Ursolic acid had good linearity in the range of 0.071 4 mg/ml to 4.284 mg/ml with r=0.999 7.The recoveries was 101.66%,RSD=1.70%. [Conclusion]The method has good precision with accurate and reliable results.
出处
《职业与健康》
CAS
2011年第20期2316-2318,共3页
Occupation and Health
关键词
高效液相色谱法
山楂
熊果酸
含量测定
High-performance liquid chromatography（HPLC）
Hawthorn
Ursolic acid
Determination
