梅毒血清学检测3种方法的对比分析

Comparative Analysis of Three Methods for Serological Detection of Syphilis

目的比较快速血浆反应素(RPR)环状卡片试验、梅毒螺旋体颗粒凝集试验(TPPA)和酶联免疫吸附法(TP-ELISA)在梅毒血清学筛查中的应用价值。方法对800份娱乐场所女服务员血清标本同步采用RPR、TPPA、TP-ELISA3种方法进行梅毒血清学检测并对比分析。结果在800份血清标本中RPR阳性6例,TPPA阳性22例,TP-ELISA阳性24例,且TP-PA与TP-ELISA相关性较高。结论 TP-ELISA可作为梅毒血清学筛查的较理想方法。

[Objective]To compare the application values of rapid plasma regain（RPR） circle card test,Treponema pallidum particle agglutination（TPPA） test and enzyme-linked immunosorbent assay（TP-ELISA） in the serological screening for syphilis. [Methods]The serological detection of syphilis was conducted in 800 serum samples which were collected from waitresses in entertainment places with RPR,TPPA and TP-ELISA simultaneously,and the results were compared and analyzed. [Results]Among 800 serum samples,6 cases were positive in RPR,22 were positive in TPPA,and 24 were positive in TP-ELISA.The correlation between TPPA and TP-ELISA was high. [Conclusion]TP-ELISA is an ideal method of serological screening for syphilis.