不同的游离脂肪酸对人乳腺癌细胞凋亡和侵袭的影响

Effects of free fatty acids on apoptosis and migration of human breast cancer cells

目的探讨不同游离脂肪酸（FFAs）对MDA-MB-231人乳腺癌细胞凋亡和迁移的影响。方法用50、200、500umol／L三种不同浓度的油酸（OA）和棕榈酸（PA）分别刺激MDA—MB-231细胞，采用实时定量PCR分析细胞中PTEN的mRNA含量，蛋白免疫印迹法检测PTEN和Bcl-2的蛋白表达，原位末端标记法（TUNEL）观察细胞的凋亡情况，小室迁移实验测定细胞的迁移能力。结果OA使MDA-MB-231细胞中PTEN的mRNA和蛋白表达水平降低，Bcl-2蛋白表达升高（P均〈0．05）；PA则。使PTEN的mRNA和蛋白表达水平上升，Bcl-2蛋白表达下降（P均〈0．05）。显微镜下观察到PA刺激后的MDA-MB-231细胞凋亡增加。迁移实验显示OA和PA都能使乳腺癌细胞侵袭能力增强。结论PA可能通过上调PTEN的表达促进MDA-MB-231人乳腺癌细胞凋亡，但同时也增强细胞的侵袭能力。OA也可增强癌细胞侵袭能力。

Objective To observe the effects of free fatty aids （FFAs） on the apoptosis and migration of human breast cancer cells （ MDA-MB-231 ）. Methods Breast cancer cells were exposed to oleic acid （OA） and palmitic acid （PA） with different concentrations （50,200, and 500 umol/L）. Quantity RT-PCR was conducted to detect mRNA expression of PTEN, and Western blot to detect protein expression of PTEN and Bcl-2. Cell apoptosis was measured with TUNEL method, and migration measured with transwell method. Results The mRNA and pro- tein expressions of PTEN in MDA-MB-231 cells were down-regulated after OA stimulation, but up-regulated after PA stimulation （ all P 〈 0.05 ）. The protein expression of Bcl-2 increased after OA stimulation and decreased after PA stimulation （ both P 〈 0. 05）. PA increased the apoptosis, and both OA and PA enhanced the invasion of MDA-MB-231 cells. Conclusions PA may promote apoptosis of MDA-MB-231 cells through changing the expres- sion of the tumor suppressor PTEN, but also increases the invasion of the cancer cells. The invasion of cancer ceils could be enhanced by OA stimulation as well.