血清25（OH）D测定的液相色谱质谱法

Liquid chromatography-mass spectrometry for measurement of serum 25 （ OH ） D level

维生素D缺乏与多种疾病相关，血清25（OH）D水平可以作为维生素D营养状况的客观指标。液相色谱质谱法（LC—MS）是临床营养常规测量25（OH）D的标准方法。本文总结了检测25（OH）D2和25（OH）D3的意义，比较了各种25（OH）D的检测方法及其优缺点，介绍了我国采用美国国立标准技术研究院质控品标准的LC-MS法常规检测25（OH）D3和25（OH）D3的情况。

Many diseases are associated with vitamin D deficiency. Serum 25 （OH）D levels can serve as objective indicators of the nutritional status of vitamin D. Liquid chromatography-mass spectrometry （LC-MS） method may be applied for routine measurement of 25 （OH） D levels in clinical practice. This article reviewed the signifi- cance of detecting 25 （OH） D2 and 25 （OH） D3, compared the advantages and disadvantages of different 25 （OH） D measurement methods, and introduced the application of LC-MS method for routine detection of serum 25 （OH）D2 and 25 （OH）93 levels following the standard by National Institute of Standards and Technology of America.