摘要
目的探讨丙烯酰胺(AA)对PC12细胞的细胞毒性及可能机制。方法分别以0、62.5、125、250、500、1 000μmol/L AA为染毒剂量,染毒12 h后,采用MTT比色法检测丙烯酰胺对体外培养PC12细胞的增殖抑制作用,并用碱性单细胞凝胶电泳技术(single cell gel electrophoresis,SCGE)检测PC12细胞DNA的损伤作用、用MDA和T-AOC试剂盒检测细胞MDA和T-AOC改变。结果染毒12 h后,细胞生长受到明显的抑制,且染毒剂量越高,抑制作用越明显(P<0.01);单细胞凝胶电泳实验表明,AA对体外培养PC12细胞的DNA有明显的损伤作用,细胞上清液中MDA含量随着染毒量的上升而升高,T-AOC含量随染毒量的上升而降低。结论 AA能够显著抑制PC12细胞活性,损伤细胞DNA,并诱导细胞氧化损伤。
Objective To explore the cytotoxicity of acrylamide（AA） to PC12 cells and the possible mechanism. Methods Cells were exposed to 0,62.5,125,250,500,and 1,000 μmol/L AA,respectively,and after 12 hrs,the MTT method was used to detect the rate of cell proliferation and inhibition,alkaline single-celled gel electrophoresis technology was harnessed to obtain the result of DNA damage level of PC12 cells,and the MDA and T-AOC kit were applied to determine the content change of MDA and T-AOC in cell cultures. Results After 12 hrs AA administration,the growth of cells was significantly inhibited.Single-celled gel electrophoresis experiment showed that the DNA of PC12 cells exposed to AA was significantly damaged.MDA content in cell culture was elevated with AA dose rising,while T-AOC content was reduced with AA dose rising.And the aforementioned toxic effect was statistical significance（P〈0.01）. Conclusions AA can significantly inhibit cell activity,cause DNA damage,and induce cells oxidative damage in PC12 cells.
出处
《实用预防医学》
CAS
2011年第10期1826-1829,共4页
Practical Preventive Medicine
基金
衡阳市社会发展科技支撑计划项目(2009ks31)
