儿童慢性HBV感染E-选择素血浆水平及基因多态性

Correlation between plasma E-selectin level and gene polymorphism in children with chronic HBV infection

目的:探讨E-选择素(E-selectin)基因第2号外显子G98T和第4号外显子A561C多态性及血浆可溶性水平与儿童慢性HBV感染的相关性。方法:采用聚合酶链反应-限制性片段长度多态性分析技术(PCR-RFLP)检测119例慢性HBV感染患儿和141例健康对照者E-选择素基因多态性,同时采用酶联免疫吸附实验(ELISA)检测各组可溶性E-选择素(sE-选择素)水平。结果:血浆sE-选择素水平在慢性HBV感染组与对照组相比差异有统计学意义(P<0.01);E-选择素G98T和A561C多态性中基因型频率和等位基因频率在慢性HBV组与对照组相比差异无统计学意义,各组中C等位基因携带者血浆sE-选择素水平明显高于A等位基因携带者(P<0.05)。结论:E-选择素G98T和A561C基因多态性在慢性HBV感染中不起作用,但A561C基因多态性参与调控血浆可溶性E-选择素的表达。

Objective：To explore the correlation between polymorphisms of G98T in the second exon,A561C in the fourth exon of E-selectin gene,plasma soluble E-selectin level and chronic hepatitis B virus（HBV） infection in children.Methods：PCR-RFLP was used to detect E-selectin gene polymorphism in 119 children with chronic HBV infection and 141 healthy children（control group）,at the same time,ELISA was used to detect the levels of soluble E-selectin in the two groups.Results：There was significant difference in the plasma soluble E-selectin level between chronic HBV infection group and control group（P0.01）.There was no significant difference in the frequencies of genotypes and alleles in E-selectin A561C and G98T gene polymorphisms between chronic HBV infection group and control group;in the two groups,the plasma soluble E-selectin level in children with C allele was significantly higher than that in children with A allele（P0.05）.Conclusion：E-selectin A561C and G98T gene polymorphisms may be not associated with chronic HBV infection,but A561C gene polymorphism participates in the regulation of plasma soluble E-selectin expression.