产后子宫内膜炎患牛致病性真菌的研究

Study of pathogenic fungi from the sickcows with postpartum endometritis

为探讨致病性真菌感染在奶牛子宫内膜炎中的作用,试验选用产后7～10 d子宫内膜炎患牛,通过真菌分离培养和套式PCR反应检测子宫真菌感染情况,并确定致病性真菌种类。结果表明:子宫内膜炎患牛真菌检出率一年四季均不同,春季为10.6%、夏季为15.2%、秋季为21.1%、冬季为18.1%,分离到的致病性真菌经鉴定为念珠球菌。说明部分子宫内膜炎患牛由致病性真菌引发,以秋冬两季真菌性子宫内膜炎发病率最高,致病性真菌主要为念珠球菌。

To investigate the effect of pathogenic fungi infection on cow endometritis, the experiment selected the cows with endometritis on postnatal day 7 to 10, and detected fungus infection in uterus, determining the type by using isolated culture, nested PCR. The result showed that the detection rate of fungi of the cows with endometritis accounted for 10.6% in spring, 15. ：2% in summer, 21. 1% in autumn and 18. 1 % in winter. The isolated fungi were Candida species. These results indicated that some of the cows with endometritis were caused by Candida infection. The infection was more in autumn and winter. The Candida could cause acute suppurative endometrltis.