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蜜蜂残翼病RT-PCR快速检测方法的建立 被引量：3

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摘要 为了建立蜜蜂残翼病病毒(Deformed wing virus,DWV)RT-PCR快速检测方法,试验采用GenBank公布的蜜蜂残翼病病毒全基因序列设计1对特异性引物,对所提取的RNA进行反转录合成cDNA后,进行聚合酶链式反应,并对这种快速检测方法的特异性和敏感性进行研究。结果表明:在约702 bp处出现目的条带,其他病原无目的条带出现,灵敏性可达1×104的模板稀释度。
作者 薛力刚 王振国 宋战昀 周亮 刘金华
机构地区 吉林农业大学发展学院 吉林出入境检验检疫局 吉林农业大学
出处 《黑龙江畜牧兽医》 CAS 北大核心 2011年第10期91-93,共3页 Heilongjiang Animal Science And veterinary Medicine
基金 国家质检总局资助项目(2005IK052)
关键词 蜜蜂残翼病 RT—PCR 检测
分类号 S895.13 [农业科学—特种经济动物饲养]
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参考文献10

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同被引文献34

  • 1Francis R M, Nielsen S L, Kryger P. Varroa-virus interaction in col- lapsing honey bee colonies[J]. PLoS One, 2013,8(3) e57540.
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  • 4Barriga G P,Cifuentes-Mufloz N,Rivera P A, et al. First detec- tion and complete genome sequence of deformed wing virus in Chilean honeybeesJ]. Virus Genes, 2012,45 (3) : 606-609.
  • 5Fernandez N, Buddrus L, Pifieiro D, et al. Evolutionary con- served motifs constrain the RNA structure organization of pi- cornavirus IRES[J], FEBS Lett,2013,587(9) :1353 1361.
  • 6Buawangpong N, Khongphinitbunjong K, Chantawannakul P. Tropilaelaps mercedesae :does this honey bee brood mite para-site exhibit a sex preference when infesting brood of the adapted host Apis dorsata ? [J]. J Apicul, 2013,52 (3) = 158-159.
  • 7Fievet J, Tentcheva D, Gauthier L, et al. Localization of de formed wing virus infection in queen and drone Apis mellifera L]-J. J Virol,2006(3):16 -17.
  • 8Martin Stephen I, Ball Brenda V, et aL The role of deformed wing virus in the initial collapse of varroa infested honey bee colonies in the UK[J]. J Apicul Res,2013,52(5) :251-258.
  • 9Chen Y P, Higgins J A, Feldlaufer M F. Quantitative real-time reverse transcription-PCR analysis of deformed wing virus in fection in the honeybee(Apis mellifera L. ) [J]. Appl Envir Mierobiol,2005,71(1) :436-441.
  • 10Farooqui T. A potential link among biogenic amines based pes- ticides,learning and memory, and colony collapse disorder: A unique hypothesis[J]. Neuro Chem Int,2013(62) :122-136.

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黑龙江畜牧兽医
2011年 第10期

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