海风藤提取物对AD模型大鼠学习记忆的影响

Effect of Piper Kadsura Ohwi on learning and memory of Alzheimer＇ s disease model in rat

目的 研究寡聚体Aβ1-42与纤丝状Aβ1-42对大鼠行为学不同程度的影响及应用海风藤干预后AD模型大鼠行为学是否发生变化.方法 采用微渗泵向侧脑室持续灌注寡聚体Aβ1-42或纤丝状Aβ1-42法制作动物模型.术后寡聚体Aβ1-42＋海风藤组和纤丝状Aβ1-42＋海风藤组大鼠每天腹腔注射10％海风藤提取物,寡聚体Aβ1-42＋二甲基亚枫（dimethyl sulfoxide,DMSO）组和纤丝状Aβ1-42＋DMSO组大鼠每天腹腔注射2.5％ DMSO,共5周.应用水迷宫实验观察大鼠的学习记忆功能变化.结果 （1）水迷宫实验结果显示,寡聚体Aβ1-42组的平均潜伏期为（89.40±7.20）s,长于纤丝状Aβ1-42组（65.00±10.89）s;纤丝状Aβ1-42＋海风藤组潜伏期为（34.00±11.26）s,短于纤丝状Aβ1.＋DMSO组（60.6 ＋6.95）s和纤丝状Aβ1-42组；寡聚体Aβ1-42＋海风藤组的潜伏期（65.33±8.89）s,短于寡聚体Aβ1-42组和寡聚体Aβ1-42＋DMSO组（85.60±6.02）s;寡聚体Aβ1-42组大鼠经过平台位置的次数（3.00±0.71）、在靶象限的时间比率（0.23±0.02）和路程比率（0.23 ±0.04）少于纤丝状Aβ1-42组（0.25±0.01,0.26±0.03,6.00±1.58）;纤丝状Aβ1-42＋海风藤组的此三项指标多于纤丝状Aβ1-42＋DMSO组和纤丝状Aβ1-42组；寡聚体Aβ1-42＋海风藤组的此三项指标多于寡聚体Aβ1-42组和寡聚体Aβ1-42＋DMSO组.以上对比具有统计学意义（P＜0.05）.结论 寡聚体Aβ1-42对大鼠行为学的影响较纤丝状Aβ1-42对大鼠行为学的影响大；海风藤干预能部分改善Aβ1-42组大鼠的学习记忆能力.

Objectives To explore the impact of Aβ oligomer（Aβo） and fibrillar Aβ（ Aβf） on the ethology of model rat and to explore whether or not piper kadsura ohwi（PKO） can ameliorate the ethological changes.Methods AD animal model was made by continuous intracerebroventricular infusion of Aβ through mini-osmotic pump.After surgery,rats of Aβo ＋ PKO group and Aβf ＋ PKO group received intraperitoneal injection of 10%PKO everyday,rats from Aβo ＋ DMSO group and Aβf ＋ DMSO group received intraperitoneal injection of 2.5% DMSO.The treatment lasted 5 weeks.Morris water maze experiment began on the 31st day after surgery.Results （ 1 ） Acquisition trials：the escape latency was longer in Aβo group （ （ 89.40 ± 7.20 ） s ） than that in Aβf group （ （65.00 ± 10.89 ） s ） ; escape latency was shorter in Aβf ＋ PKO group （ （ 34.00 ± 11.26 ） s） than that in Aβf group and Aβf ＋ DMSO group（ （60.6 ± 6.95 ） s） ; escape latency was shorter in Aβ3o ＋ PKO group （ （ 65.33 ±8.89） s） than that in Aβo group and Aβo ＋ DMSO group （ （ 85.60 ± 6.02） s）.（ 2 ） Robe trial：the times （ 3.00 ±0.71 ） and the proportion of time （0.23 ± 0.02 ） and path（0.23 ± 0.04） spent in the target quadrant in Aβo group was less than that in Aβf group（6.00 ± 1.58,0.25 ± 0.01,0.26 ± 0.03 ） ; the three parameters in Aβf ＋ PKOgroup were more than those in Aβ3f group and Aβf＋ DMSO group; the three parameters in Aβo ＋ PKO group were more than those in Aβo group and Aβo ＋ DMSO group.Conclusions Aβ oligomer had a more severe impact on the ethology of AD model rats than fibrillar Aβ did; piper kadsura ohwi may ameliorate the ethological changes of AD model rats.