PFGE法分析多血清型副溶血性弧菌引起的食物中毒

Analysis on outbreak of food poisoning induced by Multi-serotype Vibrio parahaemolyticus with pulsed-field gel electrophoresis typing

目的:对从食物中毒样品中分离到的7株副溶血性弧菌作常规鉴定及脉冲场凝胶电泳(PFGE)同源性分析,以明确致病因子。方法:按GB/T4789.7-2008进行副溶血性弧菌分离、生化鉴定、神奈川试验、血清分型;Pulse-Net PFGE分子分型标准实验室操作方法作同源性分析。结果:7株副溶血性弧菌分成三个血清型,即O3∶K6型5株,O4∶K8型和O1∶K56型各1株,分别属于三种不同的PFGE条带型,其中6株菌神奈川试验阳性。结论:这次食物中毒可能为以O3∶K6型为主的不同克隆群副溶血性弧菌混合感染所致。

Objective：To study the molecular epidemiology and homology relationship among 7 strains of Vibrio parahaemolyticus isolated from patients samples during food poisoning outbreak with routine experiment and pulsed-field gel electrophoresis（PFGE）typing,to trace the source of infections so as to support the ascertainment of epidemic control strategy.Methods： Strains isolated from samples as mentioned above were determined according to the method of GB/T 4789.7-2008.Biochemical identification,Kanagawa test,serological typing and PFGE were applied to type of the strains.Results： 7 strains with serological typing were belonged to 3 patterns,such as 5 strains of O3∶ K6,1 strain of O4∶ K8 and O1∶ K56,respectively.6 strains with Kanagawa test were positive.And PFGE result showed 3 patterns respectively.Conclusion： It indicates that mixed infection of different clone groups of vibrio parahaemolyticus with O3∶ K6 as the main type may lead to the food poisoning in this case.