广东省东莞市2010年首例输入性登革热病毒核酸检测及分析

Detection and analysis of the nucleic acids of the first imported dengue fever case in Dongguan city in 2010

目的:对广东省东莞市2010年首例输入性登革热疑似病例的标本进行快速诊断分型并对其部分核酸序列进行测序分析。方法:采用RT-PCR、PCR、基因测序技术对血清标本中的核酸进行检测分析,同时用ELISA法检测IgM抗体。结果:RT-PCR结果表明标本为Ⅰ型登革热病毒,对PCR扩增产物测序得到了包含衣壳蛋白基因和部分膜蛋白基因的455 bp长度的序列,经Blast比对发现与马来西亚的一株登革热病毒序列(GenBank号FN429890.1)相比,仅有一个C→T核酸差异,氨基酸水平无差异。IgM抗体检测为阳性。结论:PCR技术可方便快捷的用于登革热病毒的检测及分型,同时对扩增产物测序还可了解其相应的基因变异情况。流行病学结合实验室数据表明此病例为一输入性病例。

Objective：To conduct rapid diagnosis and typing for the first imported dengue doubt fever case and do sequencing analysis on part of the nucleotide sequence.Methods： To detect and analyze the nucleic acids of the blood species by RT-PCR,PCR,and sequencing technologies,to detect the IgM antibody to dengue virus by ELISA.Results： The result of RT-PCR show that it was the type 1 dengue virus.A 455 bp length sequence including the capsid gene and part of membrane gene was obtained by sequencing.It was similar to the virus isolated from Kuala Lumpur（GenBank number：FN429890.1） by Blast comparison with only one nucleotide difference（C→T）,no difference in amino level.the IgM antibody was positive.Conclusion： PCR technology can be used in the detection and typing of dengue virus conveniently and quickly.We can know the mutation situation of dengue virus gene by sequencing the PCR production at the same time.The data of lab and epidemiology show that it was an imported dengue fever case.