跑台运动训练对apoE基因敲除小鼠高同型半胱氨酸血症及氧化应激的影响

Influences of treadmill exercises training on hyperhomocysteinemia and anti-oxidant agent in ApoE knockout mice

目的:研究跑台有氧运动训练对血浆同型半胱氨酸(Hcy)水平及氧化应激的影响,探讨其可能机制,为进一步探寻延缓高同型半胱氨酸血症(HHcy)致动脉粥样硬化的有效方法提供依据。方法:6周龄雌性apoE-/-小鼠随机分为三组:对照组、HHcy组和HHcy+有氧运动组。饮用水中加入Hcy(1.8g/L)制作HHcy模型。HHcy+有氧运动组在1周适应性训练后进行8周跑台训练(0°,15m/min,60min/d,每周训练5天,间隔2天)。采用酶法检测血浆Hcy及血脂水平。羟胺法试剂盒检测血浆超氧化物歧化酶(SOD)活性。结果:与对照组相比,HHcy组血浆Hcy水平明显增高(P=0.001),HHcy+有氧运动组血浆Hcy水平较HHcy组明显下降(P=0.016)。三组之间体重增长,饮水量及血浆总胆固醇、低密度脂蛋白、高密度脂蛋白、甘油三酯水平差异无显著性意义。HHcy组小鼠血浆SOD活性较对照组显著降低(P=0.014),而有氧运动使HHcy小鼠血浆SOD活性明显增高(P=0.035)。结论:有氧运动可以降低HHcyapoE-/-小鼠血浆同型半胱氨酸水平,延缓同型半胱氨酸血症,上调氧化应激因子表达水平,改善氧化应激水平,而且该作用独立于血脂水平的改变。

Objective：To investigate the influences of aerobic exercise on hyperhomocysteinemia（HHcy） and superoxide dismutase（SOD） activities in apoE-/-mice and to explore its mechanism.Method：Six-week old female apoE-/-mice were assigned to three groups：control group,HHcy group and HHcy＋exercises group.HHcy animal model was made by feeding high Hcy chow（1.8g/L in water）.After 1 week of acclimatization,HHcy＋exercises group was trained on a motorized rodent treadmill for 8 weeks（speed：15m/min,slope：0°,60min/d,5d/week）.Plasm Hcy level and lipid level were measured enzymatically by auto-biochemistry analysis system.Plasm SOD activities were determined by hydroxylamine assay kit.Result：Plasm Hcy level in HHcy group were significantly higher than that in control group（P=0.001）,Hcy level decreased significantly in HHcy＋exercises group compared with HHcy group（P=0.016）.There was no significant difference in body weight,daily drinking amount,plasm total cholesterol,LDL-C,HDL-C and triglyceride concentrations in three groups.Compared with control group,plasm SOD activities were lower in HHcy group（P=0.014）;however,it increased in HHcy＋exercises group significantly（P=0.035）.Conclusion：Aerobic exercise could decrease plasm Hcy level and up-regulate the expression of anti-oxidant agent in HHcy apoE-/-mice,which does not depend on the decrease of cholesterol level