同种异基因造血干细胞移植急性移植物抗宿主病小鼠模型的制作

Construction of murine EL9611 erythroleukemia and acute GVHD animal model

目的制作同种异基因造血干细胞移植急性移植物抗宿主病（GVHD）Ib鼠模型。方法以C57BL／6（H_2^b）小鼠为供者，Balb／c（H-2A^4）小鼠为受者，进行同种异基因骨髓移植。设立全身照射（TBI）对照组（4只）、GVHD组（10只）、单纯骨髓移植组（10只）及正常对照组（4只）。TBI对照组仅进行致死性TBI，TBI后不进行骨髓移植；GVHD组于TBI前5d开始饮用含320mg／L庆大霉素和250mg／L红霉素的饮用水，移植当天以。。Coy射线行一次性TBI，总剂量8．0Gy，TBI后5h内每只小鼠经尾静脉输注C57BL／6小鼠骨髓细胞2×10^6个＋脾细胞1×10’个；单纯骨髓移植组预处理与GVHD组相同，每只小鼠经尾静脉输注C57BL／6小鼠骨髓细胞2×10“个。移植后观察小鼠的精神状态、活动能力、体位改变、皮毛、体重和大便等，记录每只小鼠的存活时问，计算存活率，并绘制生存曲线。濒死小鼠的皮肤、肝脏、小肠和骨髓行病理检查。结果TBI对照组小鼠的存活时间为（9．0±0．7）d，GVHD组为（32．0±3．2）d，单纯骨髓移植组为（17．5±1．6）d，3组间两两比较，存活时间的差异均有统计学意义（P〈（）．01）。TBI对照组病理检查显示造血功能衰竭。GVHD组于移植后第10～13天出现急性GVHD表现，其皮肤、肝脏和小肠组织的病理表现均符合I～Ⅱ度急性GVHD改变，单纯骨髓移植组也于移植后第10～13天出现GVHD表现，但其GVHD表现和组织学改变明显轻于GVHD组，仅为0～I度GVHD。结论Ba｜b／c小鼠经致死性TBI后移植同种异基因小鼠骨髓细胞＋脾细胞可成功制作稳定的急性GVHD模型。

Objective To construct the murine allogeneic acute GVHD model. Methods C57BL/6 （H-2b） mice were used as the donors and Balb/c （H-2d） mice as the recipients in allogeneic bone marrow transplantation （BMT）. Groups were set as total body radiation （TBI） control group （n = 4）, GVHD group （n = 10）, simple BM transplantation group （n = 10） and normal control group （n = 4）. For TBI control group, mice were subjected to TBI but did not receive BMT after radiation. For GVHD group, 5 days before TBI, gentamycin （320 mg/L） and erythromycin （250 mg/L） were added into the drinking water, and on the day of transplantation, mice received one total dose of 8. 0 Gy 80Coy TBI, and within 5 h, 2 × 10^6 C57BL/6 BM cells and 1 x 10^7 C57BL/6 spleen cells were transfused per mouse via the tail vein. For simple BMT group, the pretreatment was the same as GVHD group, and mice received only 2 x 10^6 C57BL/6 BM cells per mouse via the tail vein. The mental status, activity, posture, fur, weight, and stool were observed after transplantation. Survival time of each mouse was recorded, survival rate was calculated, and survival curve was drawn. Pathological examination was done for the liver, skin, small intestine and BM on the brink of death. Results The median survival time （MST） in TBI control group, GVHD group and BMT group was （9.0± 0. 7）, （32. 0 ± 3.2） and （17. 5 ±1.6） days respectively, and there was significant difference between every two groups （P 〈0. 01 ）. Pathological examination in TBI control group showedhematopoiesis exhaustion. GVHD group showed acute GVHD symptoms 10-13 days after allo-BMT, and the pathological changes of the skin, liver and small intestine corresponded to those of I to l] degree of GVHD. Simple BMT group also showed acute GVHD symptoms 10-13 days after alloBMT, but their GVHD manifestation and histological changes were less serious and only 0 to I degree of GVHD could be seen. Conclusion Stable acute GVHD model can be constructed by transfusion of allogeneie BM cells and spleen cells into Balb/c mice after lethal TBI.