p16及pRb在宫颈癌中的表达及意义

Immunochemical analysis of p16 and pRb in uterine cervical lesions

目的探讨人乳头瘤病毒、p16及pRb在宫颈癌的表达及其相关性。方法宫颈上皮内瘤样变(cervicalintraepithelial neoplasia,CIN)Ⅰ型患者108例,CINⅡ型患者50例,CINⅢ型患者35例及宫颈癌患者52例,应用表面等离子体谐振技术、免疫组织化学方法检测人乳头瘤病毒P16及pRb的表达情况。结果从CIN到宫颈癌患者人乳头瘤病毒、P16阳性率呈逐渐增高趋势,pRb阳性率呈逐渐下降趋势;P16表达与pRb表达呈负相关(r=-0.537,P<0.01),人乳头瘤病毒与pRb表达呈负相关(r=-0.513,P<0.05),人乳头瘤病毒与P16表达呈正相关(r=0.815,P<0.05)。结论人乳头瘤病毒感染是宫颈癌发生的主要原因,p16可作为宫颈癌的早期预测指标。

Objective To detect the expression of p16 and pRb in cervical intraepithelial neoplasia and their association with cervical cancers.Methods Human papilloma virus was detected with surface plasmon resonance technique,and the expressions of P16 and pRb were examined with immunohistochemistry technique in 108 cases of CINⅠ,50 cases of CINⅡ,35 cases of CINⅢ and 52 cases of cervical carcinomas.Results The positive rate of human papilloma virus and P16 showed increasing tendency,and the positive rate of pRb showed decreasing tendency in CINⅠ,CINⅡ,CINⅢ,cervical squamous carcinoma and cervical adenocarcinoma.The expression of P16 and human papilloma virus were negatively correlated with the expression of pRb（r=-0.537,P0.01;r=-0.513,P0.05）,and the expression of human papilloma virus was positively correlated with the expression of P16（r=0.815,P0.05）.Conclusion Human papilloma virus infection is an essential factor of cervical cancer.The expression of p16 would be an early indicator for cervical cancer.