甲泼尼龙对脊髓损伤大鼠氧自由基的影响

Prophylactic effect of high-dose methylprednisolone on oxygen free radical in rats with acute spinal cord injury

目的了解急性脊髓损伤大鼠预防应用大剂量甲泼尼龙后氧自由基的变化。方法 40只SD大鼠随机分为观察组和对照组各20只,利用Allen重物打击造成脊髓损伤,于损伤前30 min,观察组预防应用大剂量甲泼尼龙,对照组应用生理盐水。于脊髓损伤后24,72 h分别进行神经功能评分及检测超氧化物歧化酶和丙二醛水平。结果损伤后24 h,观察组与对照组Tarlov功能评分比较差异无统计学意义(P>0.05),72 h时,观察组较对照组明显提高(P<0.05);脊髓损伤后24,72 h观察组大鼠血清和损伤段脊髓中超氧化物歧化酶较对照组增高(P<0.01),丙二醛较对照组降低(P<0.01);观察组超氧化物歧化酶及丙二醛在损伤后72 h与24 h相比较,差异均有统计学意义(P<0.01)。结论预防应用大剂量甲泼尼龙可促进急性脊髓损伤大鼠氧自由基清除,保护神经功能。

Objective To investigate the prophylactic effect of high-dose methylprednisolone on oxygen free radical in rats with acute spinal cord injury.Methods Forty SD rats were randomly divided into observation group and control group with 20 rats in each group.The observation group received high-dose methylprednisolone and control group received normal saline 30 minutes before acute spinal cord injury induced by Allen＇s weight drop.Tarlov motor scale,superoxide dismutase and malondialdehyde were detected 24 and 72 hours after spinal cord injury.Results There was no significant difference in Tarlov motor scale 24 hours after spinal cord injury between two groups.Tarlov motor scale was significantly higher 72 hours after spinal cord injury in the observation group than that in control group.Superoxide dismutase level was obviously higher and malondialdehyde level was lower 24 hours and 72 hours after spinal cord injury in the observation group than that in control group.There were significant differences in superoxide dismutase and malondialdehyde levels between 24 hours and 72 hours in the observation group（P0.01）.Conclusion High-dose methylprednisolone can clear oxygen free radical and protect brain nerves in rats with acute spinal cord injury.