S100a7蛋白在体外培养人视网膜色素上皮细胞中的表达及功能

Expression identification and preliminary function analysis of S100a7 in retinal pigment epithelial cells cultured in vitro

背景:S100a7蛋白在细胞增殖、血管形成等病理生理过程中发挥重要作用,但在视网膜色素上皮细胞中的作用少有报道。目的:探讨S100a7蛋白在体外培养人视网膜色素上皮细胞中的表达及作用。方法:体外培养ARPE-19细胞株,通过免疫荧光及Western blot技术,检测S100a7在ARPE-19细胞中的表达;不同稀释度(1∶1000,1∶10000,1∶100000)S100a7蛋白抗体与ARPE-19细胞共培养,通过MTT技术检测S100a7蛋白在人视网膜色素上皮细胞增殖过程中的作用。结果与结论:免疫荧光及Westernblot技术证实该蛋白在ARPE-19细胞中表达;MTT实验结果显示,人视网膜色素上皮细胞加入S100a7抗体72h后,各浓度处理组的吸光度值较对照组显著均降低(P<0.05)。结果证实S100a7蛋白在人视网膜色素上皮细胞中的表达,并明显促进人视网膜色素上皮细胞增殖。

BACKGROUND：S100a7 is a new protein that participates in cell proliferation, vascularization and other pathophysiological processes. OBJECTIVE：To discuss the expression of S100a7 in retinal pigment epithelial cell （RPE） and to investigate the effects of S100a7 on RPE proliferation. METHODS：Immunofluorescence and western blot techniques were used to detect the expression of S100a7 in ARPE-19, and MTT was performed to investigate the effects of S100a7 on RPE proliferation. RESULTS AND CONCLUSION：S100a7 was proven to be expressed in ARPE-19 by immunofluorescence and western blot techniques and it could significantly promote ARPE-19 cell proliferation. This study first confirmed the expression of S100a7 in ARPE-19 cells, which may play a specific role in the pathogenesis of proliferative vitreoretinopathy.