不同浓度姜黄素对体外培养小胶质细胞过度活化的抑制作用

Inhibitory effects of different concentrations of curcumin on excessive activation of microglia cultured in vitro

背景:过度活化的小胶质细胞会释放大量的神经毒性因子损伤神经功能。目的:观察不同浓度的姜黄素对体外培养小胶质细胞过度活化的抑制作用。方法:取新生48h内Wistar大鼠用于小胶质细胞的提取及原代培养,分别加入1,5,20μmol/L的姜黄素抑制3,7d,观察细胞形态,CD11b/c的表达,Notch1mRNA的表达。结果与结论:原代培养的小胶质细胞折光性强,以圆形多见,呈过度活化状态。姜黄素干预后静止细胞阳性率明显升高,在培养第7天时阳性率达到高峰。RT-PCR结果显示,1,5,20μmol/L姜黄素都可明显抑制小胶质细胞Notch1 mRNA的表达。以5μmol/L姜黄素的抑制效果最显著(P<0.01)。说明姜黄素对体外培养的小胶质细胞过度活化的抑制作用存在剂量依赖性。

BACKGROUND：Excessively activated microglia can release a large number of neurotoxic factors which damage nerve function. OBJECTIVE：To investigate the inhibitory effects of different concentrations of curcumin on excessive activation of microglia cultured in vitro. METHODS： Microglias were extracted from neonatal （within 48 hours） Wistar rats and cultured. At 3 and 7 days after addition of different concentrations of curcumin （1, 5, 20 μmol/L）, cell morphology, CD11b/c expression and Notch1 mRNA expression were determined. RESULTS AND CONCLUSION：The primary cultured microglia exhibited strong refractive capacity and a spherical morphology and were excessively activated. After intervention of curcumin, the positive rate of static cells was obviously increased, and the positive rate reached a peak on day 7 of culture. RT-PCR showed that 1, 5, 20 μmol/L curcumin can obviously inhibit Notch1 mRNA expression in the microglia, and 5 μmol/L curcumin showed the most obvious inhibitory effects （P 0.01）. These results suggest that curcumin shows a dose-dependent inhibitory effect on excessive activation of microglia cultured in vitro.