壮筋续骨汤含药血清体外培养大鼠成骨细胞的增殖

Effects of Zhuangjinxugu decoction-containing serum on the proliferation of rat osteoblasts cultured in vitro

背景:壮筋续骨汤具有促进胫骨骨折愈合的作用。目的:观察壮筋续骨汤含药血清对体外培养大鼠成骨细胞增殖的影响。方法:大鼠灌胃给予壮筋续骨汤后制备含药血清。取第2代生长状况良好出生48h内的SD大鼠成骨细胞,对照组和壮筋续骨汤组分别加入5%,10%,20%的正常SD大鼠血清和含药血清培养72h。结果与结论:MTT法检测发现壮筋续骨汤含药血清对成骨细胞的增殖有明显的促进作用,以10%壮筋续骨汤组作用最明显(P<0.05)。流式细胞仪检测各组细胞DNA合成期(S期)的比例,发现壮筋续骨汤组处于S期的细胞比例明显大于对照组(P<0.05)。说明壮筋续骨汤含药血清能促进成骨细胞DNA的合成,使更多的细胞进入S期,促进体外培养的成骨细胞增殖。

BACKGROUND：Zhuangjinxugu decoction has been shown to promote the healing of tibial fracture. OBJECTIVE：To investigate the effects of Zhuangjinxugu decoction-containing serum on proliferation of rat fibroblasts cultured in vitro. METHODS：Rats were intragastrically administered with Zhuangjinxugu decoction to prepare drug-containing serum. Passage 2 well growing SD rat fibroblasts were harvested within 48 hours after rat birth. Rats from the control and Zhuangjinxugu decoction group were intragastrically administered with 5%, 10%, 20% normal SD rat serum and Zhuangjinxugu decoction-containing serum and then cultured for 72 hours. RESULTS AND CONCUSION：Cellular proliferation in each group was detected by MTT and results showed that Zhuangjinxugu decoction-containing serum greatly promote the proliferation of fibroblasts, in particular 10% Zhuangjinxugu decoction-containing serum （P 0.05）. The proportion of cells at DNA synthesis （S stage） was detected by flow cytometry, and the proportion of cells at the S stage was greater in the Zhuangjinxugu decoction group than in the control group （P 0.05）. These findings suggest that Zhuangjinxugu deconction-containing serum can promote DNA synthesis in the fibroblasts, which makes more cells entering into S stage and promotes the proliferation of fibroblasts cultured in vitro.