少年男性快肌纤维预测方程的建立

Establishment of a speculative equation for fast twitch muscle fiber in junior sportsmen

背景:三磷酸腺苷酶染色法是直接测定肌纤维类型的方法之一,但测量技术繁琐,难以进行大样本测量。目的:拟建立用于检测的快肌纤维推测方程。方法:纳入广西各地、市体校少年男子运动员229例为受试对象,随机分为方程建立组160例、验证组39例、推广组30例。299例受试对象三磷酸腺苷酶染色法的测量结果与刘瑞莲、岳君方程的推测结果进行比较;建立组以三磷酸腺苷酶染色法的测量的快肌纤维百分比数据为因变量,以部分素质、机能指标数据自变量,采用逐步多元回归分析,建立快肌纤维百分比推测方程。结果与结论:少年男性快肌纤维百分比推测方程为:FT%=10.5617-9.0320×(60m跑,s)-0.1690×(原地纵跳摸高,cm)+0.3135×(最高无氧功率,kg·m/min);验证组回代验证的结果显示与三磷酸腺苷酶染色法测量结果平均值之间差异无显著性意义;推广组回代验证的结果显示与验证组三磷酸腺苷酶染色法测量结果平均值之间差异无显著性意义。说明快肌纤维预测方程具有较高的准确性与科学性,可以应用该方程对青少年快肌纤维百分比进行推测。

BACKGROUND：ATPase staining is one of methods used for direct determination of muscle fiber type, but the measurement procedure is tedious and can be hardly used for large sample size measurement. OBJECTIVE：To establish a speculative equation for fast twitch muscle fiber in junior sportsmen. METHODS：A total of 229 subjects aged 13-17 years from different Guangxi regions and Sports School in Qinzhou were included in this study and were randomly divided into three groups：equation （n=160）, testing （n=39） and generalization （n=30）. The measurement results of ATPase staining from 299 subjects were compared with those from Liu Rui-lian and Yue Jun. The stepwise regression analysis was performed taking the percentage of fast twitch muscle fiber measured by ATPase staining as the dependent variables and taking partial body quality and functional indexes as the in independent variables to establish the speculative equation of fast twitch muscle fiber. RESULTS AND CONCLUSION： The speculative equation on fast twitch muscle fiber in junior sportmen was as follows：FT% = 10.561 7-9.032 0×（60-meter running, s） -0.169 0×（in situ jump height, cm） ＋ 0.313 5× （the highest anaerobic power, kg·m/min）. There were no significant differences between the checking results in testing groups and the average means of ATPase staining （P=0.150 0）, and ATPase staining also had no significant difference with the verifying results in the generalization group （P=0.234 0）. Results showed that the speculative equation for fast twitch muscle fiber in junior sportsmen is highly accurate and scientific.