嗜酸乳杆菌的表面疏水性分析

Analysis of the surface hydrophobicity of Lactobacillus acidophilus

对嗜酸乳杆菌的表面疏水性进行分析。利用微生物粘着碳氢化合物法测定嗜酸乳杆菌的表面疏水率,通过采用不同环境条件和胰蛋白酶处理菌体细胞表面,初步确定嗜酸乳杆菌表面疏水性的影响因素。结果表明,影响菌体表面疏水性的因素有时间、温度、pH值、浓度、Ca2+和胰蛋白酶,菌体表面疏水性具有时间、温度、pH值和浓度依赖性,Ca2+和胰蛋白酶对其有一定作用,且表面疏水性与黏附能力之间存在着相关性;此外,推测能够介导嗜酸乳杆菌表面疏水性的某些物质可能是一类蛋白质。

We analyzed the surface hydrophobicity of Lactobacillus acidophilus. We utilized the method of microbial adhesion the hydrocarbons to determine the surface hydrophobic rate of Lactobacillus acidophilus and used the different environmental conditions and trypsin to process the cell to determine the influencing factors of the surface hydrophobicity of Lactobacillus acidophilus preliminary. Through this research, we ob- tained that the influencing factors of the surface hydrophobicity of Lactobacillus acidophilus were time, temperature, pH, concentration, Ca2＋ and trypsin. Cell surface hydrophobicity had the dependent manner of time, temperature, pH and concentration, and was affected by the Ca2＋ and trypsin, and was connected with the adhesion ability. In addition, we speculated that some substances which could mediate the hydrophobicity of Lactobacillus acidophilus may be a class of proteins.