调制乳中8种合成色素的快速测定

Quickly determination of eight kinds of synthetical pigmentsin blend milk

以乙腈溶液沉降蛋白并提取样品中人工合成色素，应用反相高效液相色谱－多波长紫外检测器，外标法定量，测定了调制乳中8种人工合成色素的质量浓度。8种人工合成色素质量浓度在0．1～10．0mg／L范围内呈良好线性关系，r=0．9999，平均加标回收率为81．7％～106．0％，CV=0．77％～8．97％。该方法简便，准确，重现性好，可用于调制乳中8种合成色素质量浓度的测定。

Protein in blend milk was coagulated by CH3CN and eight kinds of synthetical pigments in blend milk was purified , the solution was determined by HPLC-DAD. Results showed the linearity of eight kind of synthetical pigments was in the range of 0. 1 -10.0 mg/L, r = 0.9999. The average recovery was in the range of 81.7 % - 106.0 %, CV was between 0.77 % and 8.97 %. This method was quickly and accurate with good reproducibility and can be used for the determination of eight kinds of synthetical pigments in blend milk.