阿特拉津暴露对女性生殖健康影响研究

Study of the adverse influence of atrazine exposure on the women's reproductive health

调查女性工人在阿特拉津暴露3个月前后尿液中阿特拉津及其代谢产物浓度、血液中生殖激素浓度和月经周期变化。结果表明,阿特拉津暴露3个月后,工人尿液中脱乙基脱异丙基阿特拉津和脱异丙基阿特拉津浓度分别提高了2倍和3倍,女工血液中雌激素水平有升高,同时月经和排卵周期缩短。阿特拉津暴露后女工正常生殖功能受到某些影响,说明阿特拉津类除草剂有可能是内分泌干扰物质,建议加强对阿特拉津生产工人的安全防护工作。

The paper is engaged in a study of the influence of atrazine exposure on the women＇s reproductive health. As is reported, atrazine is an herbicide widely used in many places of the world and its residues were found in the endocrine systems of amphibian insects and small creatures in different levels of exposure. However, there has been no evidence of its adverse effect on human health, which leads to a great difference of the official opinions on its application as a kind of herbicide. It is just for this reason that the present paper is devoted to a detailed investigation and assessment of the exposure for occupational women to atrazine and its effects on their reproductive health by using epidemiological methods and modem analytical tools combined. In order to make the study more accurate and purposeful, we have invited 17 volunteer female subjects from an atrazine work- shop with its airborne atrazine concentrations of 16.9 μg/m3 and 113.1 μg/m3. Urine samples and blood samples of the said volunteer subjects were inspected and analyzed with the atrazine and its chlorodealkylated metabolites in thier urine by gas chromatography. Moreover, we have also tested and measured six kinds of serum re- productive hormones by means of a chemilumineseent fluorescence immunoassay detector. Furthermore, the subjects were asked to fill in ovulation sheets for 3 months, with the menstrual and ovulation cycles registered in the sheets. The results show that, after inhaled airborne atrazine for 3 months, the urinary atrazine metabolites deisopropyla- trazine （DIA）, deethyldeisopropylatrazine （DEDIA） and deethyla- trazine （DEA） of these subjects increased significantly with DIA in- creased twice and the DEDIA--triee. The estradiol concentrations of the subjects increase from 44.6 pg/mL to 123.6 pg/mL, with no sig- nificant difference between the concentrations of androstenedione, LH, FSH, SHBG and total testosterone before and after 3 months. In addition, their menstrual cycles and ovulatory period had been short- ened after the atrazine occupational exposure. The results show that haman reproductive health had been affected after atrazine exposure, indicating atrazine may be an endocrine disruptor. Therefore, it is necessary to enhance the safety measures to better protect women workers in this line.