消化道肿瘤患者血清TK1的表达及临床意义

The expression and clinical significance of serum TK-1 in gastrointestinal tumors

目的研究评价消化道肿瘤患者血清胸苷激酶1（STK1）的表达,同时联合检测消化道肿瘤标记物CEA、CA199,探讨其在早期诊断、临床分期、治疗疗效的临床价值。方法应用化学增强发光（ECL）点印迹法定量检测150例消化道肿瘤患者治疗前后STK1浓度,酶免疫测定法CEA、CA199浓度;40例健康志愿者作为对照组。结果肿瘤患者与健康人STK1浓度范围有差别,敏感度为55%,特异性为87.5%。STK1与CEA、CA199比较有差异。而STK1＋CEA＋CA199联合检验与单独行STK1、CEA、CA199检测比较提高了近30%。Ⅰ~Ⅱ期患者STK1平均值与Ⅲ~Ⅳ期患者STK1平均值比较有差异。CR、PR、PD患者随病情的进展治疗前后STK1值逐渐升高。结论 STK1的表达明显高于正常人,与CEA、CA199联合检测能提高其在诊治中的阳性率,并且在消化道肿瘤诊断、判断预后、监测疗效等方面具有一定的临床应用价值。

Objective To evaluate the expression of thymidine kinase 1（STK1） in patients with gastrointestinal cancer and detection of CEA,CA199 of tumor markers,and explore its early diagnosis,clinical stage and clinical value of therapy.Methods The levels of STK1 in 150 patients with gastrointestinal tumors and 40 normal controls were determined by enhanced chemiluminescence assay.Results The density of STK1 in gastrointestinal tumors and healthy individuals had statistical significance.The sensitivity was 55%,and specificity was 87.5%,Compared with CEA and CA199,STK1 had obvious difference.Compared with separate STK1,CEA,CA199,the combined detection of STK1＋CEA＋CA199 increased by nearly 30%.The average density of STK1 in patients with period Ⅰ~Ⅱ and period Ⅲ~Ⅳ had obviously statistical significance.With the progress of disease,STK1 value of CR,PR,PD patients gradually increased before and after treatment.Conclusion The levels of STK1 in patients with gastrointestinal tumors were much higher than those in patients with normal control.Combined detection with CEA and CA199 can improve its positive rate.The detection of STK1 may have value in the diagnosis,monitoring and prognosis of gastrointestinal tumors.