产壳聚糖酶菌株的诱变育种及其产酶条件

Screening and Mutagenesis of Chitosanase-Producing Strain and Study on the Optimal Conditions of Chitosanase Formation

利用紫外线诱变不同稀释度的黑曲霉菌液后，筛选得到一株酶活力较强的菌株A3，其酶活为52．34nkat／mL，并对其进行了Sephadex G-200凝胶柱层析纯化。对A3发酵产酶条件的研究结果表明，最适产酶发酵条件为发酵温度范围27oC～30oc，发酵时间72h，pH6．0，摇床转速130r／min，壳聚糖含量1．0％。

A strain with relatively high ability to produce chitosanase was screened out by ultraviolet irradiation mutagenesis, chitosanase produce was 52.34 nkat/mL, purifing the enzyme with Sephadex G-200. The optimal condition producing chitosanas for A3 was as follows： the fermentation temperature was 27℃-30℃, the fermentation time was 72 h, pH value was 6.0, shaking speed was130 r/rain, and chitosan content was 1.0 %.