响应面法优化葡萄籽中原花青素提取

Optimization of the Processing Parameters for the Extraction of Procyanidine from Grape Seed Using Response Surface Methodology

在比较不同的提取方法后,选用乙醇为提取溶剂进行超声循环提取。通过单因素试验结合响应面法对葡萄籽中原花青素的提取工艺进行优化研究,确定提取工艺的最佳条件:乙醇体积分数50.9%,超声波功率1 008 W,提取时间25.3 min,料液比1∶20,提取温度50℃。在此条件下葡萄籽中原花青素提取率理论值为3.450%,实测值为(3.512±0.051)%。

Based on the results of single-factor-at-a-time-technique, a three-level Box-Behnkenfactorial design combining with response surface methodology （RSM） was employed to optimizethe extraction condition of grape seed Procyanidine.Canonical analysis was applied to estimate that a maximal extraction rate of Procyanidine 3.450 % could be obtained. The extraction conditions, such as temperature, solid-liquid ratio, extraction time, ultrasonic power and the concentration of ethanol was 50℃, 1：20, 25.3 min, 1 008 W and 50.9 %, respectively. These predicted values were also verified by validation experiments.