摘要
【目的】采用real-time PCR方法检测体外激素处理对奶牛乳腺上皮细胞FcRn(neonatal Fc receptor,FcRn)mRNA表达的影响。【方法】在乳腺上皮细胞培养液中添加不同浓度胰高血糖素(0.01、0.1和1μmol.L-1)、胰岛素(0.005、0.1和0.5μmol.L-1)、甲状腺素T4(0.01、0.1和1μmol.L-1),体外培养并收集细胞,提取细胞mRNA后,利用real-time PCR检测FcRn mRNA的相对丰度。【结果】在一定添加剂量范围内,随着添加剂量的增加,甲状腺素和胰高血糖素能够显著地促进FcRn mRNA表达量升高(P<0.05),胰岛素在添加量为0.005μmol.L-1和0.5μmol.L-1时FcRn mRNA的表达量低,但在0.1μmol.L-1添加时表达量显著升高(P<0.05)。【结论】添加外源胰岛素、胰高血糖素和甲状腺素能够影响FcRn mRNA的表达,为进一步研究乳腺内FcRn受体变化以及影响因素提供了依据。
【Objective】The mRNA expression of FcRn was studied in Holstein Cows mammary gland epithelial cells treated with hormone by real-time PCR.【Method】 FcRn mRNA expression of in vitro cultured mammary epithelial cells treated with different concentrations of thyroxine hormone（0.01,0.1,1 μmol？L-1）,insulin（0.005,0.1,0.5 μmol？L-1） and glucagon（0.01,0.1,1 μmol？L-1）,respectively,was determined.【Result】The results showed that the mRNA abundance of FcRn increased with the higher dose of thyroxine and glucagon significantly（P0.05） in a certain range.However,the mRNA expression of FcRn was significantly lower when treated with 0.005 μmol？L-1 and 0.5 μmol？L-1 insulin than 0.1μmol？L-1（P0.05）.【Conclusion】Thyroxine,insulin and glucagon can obviously change the level of FcRn mRNA,which provide a basis for further research of the change and influence of FcRn.
出处
《中国农业科学》
CAS
CSCD
北大核心
2011年第20期4279-4283,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(30871837)
