摘要
【目的】探讨胰岛素样生长因子-I(IGF-I)对产蛋鸡等级前卵泡发育的影响。【方法】用免疫组化和RT-PCR法检测IGF-I受体在鸡等级前卵泡中的表达,研究IGF-I对鸡早期卵泡发育的影响。【结果】IGF-I受体在等级前卵泡颗粒层和膜层均有表达,且随着卵泡的发育其表达水平逐渐增高,在小黄卵泡(SYF)阶段达到最大;同时,卵泡体外培养结果表明IGF-I(100 ng.mL-1)、FSH(10 n.mL-1)及IGF-I+FSH处理可显著增加SYF中膜层和颗粒层的厚度及细胞密度,且IGF受体在颗粒层和膜层中的表达水平也显著增加,其中以IGF-I+FSH联合处理组的效果最为显著。【结论】IGF-I能显著提高产蛋鸡SYF颗粒层和膜层的细胞数目以及厚度,同时增加IGF-I受体的表达,并可与FSH联合作用以提高IGF受体的表达,促进鸡卵泡细胞的增殖,进而调节鸡等级前卵泡的发育,此结果有助于揭示家禽卵泡发育的局域性内分泌调节作用。
【Objective】The aim of the experiment is to investigate the effect of IGF-I on prehierarchical follicle development from laying chickens.【Method】IGF-I receptor（IGF-IR） expression in granulosa and theca cells was studied in the prehierarchical follicles of laying chickens via immunohistochemistry and RT-PCR.In addition,the effects of IGF-I on small yellow follicle（SYF） development were investigated.【Result】The expression of IGF-IR gradually increased in both granulosa and theca layers from the prehierarchical follicles.Moreover,IGF-IR displayed the highest expression in SYF.Compared with control,treatment with IGF-I and FSH alone or in combination significantly increased cell density,thickness of granulosa and theca layers,stimulated the expression IGF-IR in both granulosa and theca layer from SYF.Furthermore,IGF-I and FSH displayed an additive effect.【Conclusion】 These results indicate that IGF-I may increase follicular cell number,thickness of granulosa and theca layers and stimulated IGF-IR mRNA expression in SYF.In addition,IGF-I exhibited synergistic effect with FSH to enhance prehierarchical follicular cell proliferation.Therefore,these results may facilitate delineating the mechanism of intra-ovarian endocrine regulation of poultry follicle development.
出处
《中国农业科学》
CAS
CSCD
北大核心
2011年第20期4295-4301,共7页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(30871843)
浙江省科技厅项目(2008C22040)
