摘要
针对阪崎肠杆菌的ompA基因设计4条特异性引物(2条内引物、2条外引物),建立一种快速检测阪崎肠杆菌的DNA环介导恒温扩增法(LAMP)。通过对2株阪崎肠杆菌标准菌株、24株阪崎肠杆菌分离株和18株非阪崎肠杆菌测试,结果表明此方法具有很高的特异性;对其灵敏度进行检验,发现该方法的最低检测限可以达到8CFU/mL,具有较好的敏感性。在1份实验室能力验证盲样和26份实际奶粉样品中应用发现,该方法与传统生化方法结果吻合,具有较好的可靠性。
Four primers including two inner primers,two outer prim-ers were designed specifically to recognize the ompA gene of E.sakazakii and a method of loop-mediated isothermal amplification was set up to detect Enterobacter sakazakii rapidly.The results sug-gested that the method had high speciality to 2 E.sakazakii typical strains,24isolates and 18non-E.sakazakii.More over,it had good sensitivity with the lowest detectable limit of 8CFU/mL.Through detecting the proficiency tests of E.sakazakii in milk pow-der and 26milk powder samples by this method,the results indicated that it had the same results as traditional biochemical method and may be used to detect samples.
出处
《食品与机械》
CSCD
北大核心
2011年第5期111-114,共4页
Food and Machinery
基金
广东省科技计划项目(编号:2008A040101001
2009B040500001)
广东省科学院青年科学研究基金项目(编号:qnjj20099)