茶叶渣不溶性膳食纤维通便及其机制研究

STUDY OF INSOLUBLE FIBER FROM TEA RESIDUE ON DEFECATION AND ITS MECHANISM

目的探讨茶叶渣中不溶性膳食纤维(TIDF)的持水和膨胀性及其润肠通便的作用。方法比较5种粒径TIDF在人工模拟无胃蛋白酶的胃液和人工模拟无胰蛋白酶的肠液中的持水力、膨胀力随时间的变化。分别以5种粒径、3个剂量的TIDF干预KM雄性小鼠,测定排便情况;以复方地芬诺酯建立便秘模型,测定黑便排出状况和墨汁推进率。结果 TIDF在人工模拟NE胃液和人工模拟NE肠液中的持水、膨胀特性均相同,持水力和膨胀力由小到大排列为:60目<80目<200目<150目<100目。5%、10%和15%的TIDF或5种目数的TIDF均可明显促进小鼠排便,显著提高便秘小鼠的小肠推进率、粪便重量和粒数、缩短首便时间(P<0.01);当TIDF为100目、干预剂量大于10%时,便秘小鼠的小肠蠕动和排便效果最佳。结论 TIDF具有较好的通便作用,剂量越大或较小粒径TIDF具备较好的持水力及膨胀力。

Objective To study the water-holding and expansive capacity of tea insoluble fiber（TIDF） and its effect on defecation.Method The changes of water-holding and expansive capacity of TIDF of 5 particle sizes in artificially simulated non-enzymatic（NE） gastric and intestinal juice were compared.Male KM mice were fed TIDF of 5 particle sizes and 3 doses,and the defecation property of the mice was determined.Using the constipation model induced by diphenoxylate,the defecation property of black feces and the percentage of ink-pushing in small intestine were determined.Results The water-holding and expansive capacity of TIDF in artificial simulation NE gastric and intestinal juice was same,and the TIDF particle size sequence based on the water-holding and expansive capacity from the least to the most was 6080 200 150 100 mesh.5%,10% and 15% TIDF or the 5 particle size TIDF could promote mice defecation,increase the percentage of ink-pushing in small intestine and feces weight and number,decrease the defecation time of first black feces significantly for the costive mice（P0.01）.The enterocinesia and defecation effect of the costive mice fed with a TIDF particle size of 100 mesh and its dose over 10% were the best.Conclusion TIDF has good improving enterocinesia and defecation function.Higher dose and smaller particle size TIDF have better water-holding and expansive capacity.