18例复发性流产患者调节性T淋巴细胞在孕期前后变化被引量：1

Change of regulatory T lymphocyte before and after pregnancy in 18 patients with recurrent abortion

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摘要目的:对18例复发性流产患者采用主动免疫方法,治疗前后进行CD4+CD25+highT细胞、CD4+T细胞、CD8+T细胞水平检测,探讨T细胞群在妊娠期参与调控母体免疫反应中的作用。方法:采用流式细胞仪分别对18例复发性流产患者在流产后初诊时、免疫治疗后3次、孕后免疫治疗2次时其外周血淋巴细胞CD4+CD25+highT细胞、CD4+T细胞、CD8+T比例进行分析。结果:18例复发性流产患者的CD8+T细胞占淋巴细胞初诊时比例为(22.5±1.35)%、免疫治疗后3次比例为(26.10±1.62)%;CD4+CD25+highT细胞占淋巴细胞比例初诊时为(0.98±0.26)%,免疫治疗后3次比例为(1.25±0.35)%;其中12例复发性流产患者孕后免疫治疗2次时的CD8+T占淋巴细胞比例为(29.50±2.20)%,CD4+CD25+highT细胞占淋巴细胞比例为(1.56±0.29)%。结论:主动免疫治疗可以提高外周血CD4+CD25+highT、CD8+T细胞水平,从而调控母胎界面免疫耐受的形成。 Objective： To detect the levels of CD4 ^＋ CD25^＋ high T cells, CD4^＋ T cells and CDs ~ T cells before and after active im- munity among 18 patients with recurrent abortion, explore the function of T cell population in regulation of maternal immune reaction during pregnancy. Methods： Flow cytometry was used to analyze the proportions of CD4^＋ CD25 ^＋ high T cells, CD4 ^＋ T cells and CD8^＋ T cells among 18 patients with recurrent abortion at first visit after abortion, after immunotherapy （3 times ） and after postpartum immunotherapy （2 times） . Results： Among 18 patients with recurrent abortion, the proportions of CDs ＋ T cells among lymphocytes at first visit after abortion and after immunotherapy （3 times） were （22. 5 ± 1.35）% and （26. 10 ± 1.62）% , respectively; the proportions of CD4^＋ CD25^＋ high T cells among lymphocytes at first visit after abortion and after immunotherapy （ 3 times） were （ 0. 98± 0. 26 ） % and （ 1.25 ± 0. 35 ） % , re- spectively. Among 12 patients with recurrent abortion receiving postpartum immunotherapy for 2 times, the proportions of CD8^＋ T cells and CD4 ^＋ CD25^＋high T cells among lymphocytes were （29. 50 ± 2. 20） % and （ 1.56 ± 0, 29 ） % , respectively. Conclusion： Active immuno- therapy can improve the levels of CD4^＋ CD25 ^＋high T cells and CDS＋ T cells in peripheral blood, then it can regulate the formation of immune tolerance on maternal -fetal interface.

作者许飞侍佳陆牡丹

机构地区江苏省无锡市妇幼保健院

出处《中国妇幼保健》 CAS 北大核心 2011年第29期4546-4547,共2页 Maternal and Child Health Care of China

基金吴阶平医学基金项目〔320.6750.09083〕

关键词复发性流产 CD4^+CD25^+highT细胞 CD4^+T CD4^+T Recurrent abortion CD4 ＋ CD25^＋high T cell CD4 ^＋ T CD8 ^＋ T

分类号 R714.4 [医药卫生—妇产科学]

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参考文献12

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