单纯及丙泊酚联合电休克对抑郁大鼠海马TNF-α表达的影响

Effects of Propofol for Depressed Rats on Hippocampus TNF-α Undergoing Electroconvulsive Therapy

目的探讨单纯电休克及丙泊酚联合电休克对抑郁大鼠电休克治疗效果及海马内TNF-α表达的影响。方法 50只成年Wistar大鼠随机分为5组(n=10):正常对照组(C组)、抑郁组(D组)、单纯丙泊酚组(DP组)、单纯电休克组(E组)、丙泊酚联合电休克组(M组)。C组正常饲养,其余采用经典的慢性不可预见性应激法建立抑郁模型。建模成功后,E组直接行电休克治疗,M组腹腔注射丙泊酚90 mg/kg,麻醉产生效应后行电休克治疗,每日进行以上处理1次,连续7日。以Open-field法和糖水消耗实验进行行为学评分。实验完成后处死大鼠,用免疫组化染色法检测TNF-α受体蛋白的表达;ELISA法海马TNF-α的含量,RT-PCR法测海马TNF-α mRNA表达。结果 E组和M组大鼠的行为学评分高于D组和DP组,且M组高于E组(P<0.05);D组和DP组海马内TNF-α含量以及mRNA的表达高于其它各组(P<0.05),M组海马内TNF-α含量以及mRNA的表达低于E组(P<0.05),与C组差异无统计学意义(P>0.05);D组和DP组海马区TNF-α受体表达量低于C组(P<0.05),M组的表达量接近于C组,但高于E组(P<0.05)。结论慢性应激使大鼠海马内TNF-α含量升高及其受体表达下调,从而产生抑郁症。丙泊酚可以改善电休克抑郁症状可能与进一步下调抑郁大鼠海马内TNF-α的含量和上调其受体表达有关。

Objectlve To observe the propofol on therapeutic effect of depressed rats undergoing electroconvulsive therapy （ECT）, Detecting the inflammatory cytokine TNF-α contents and their receptor expression in the hippocampus. Methods Fifty Wistar rats,200 - 300 g, were randomly divided into five groups, and each group was ten ： group control （ group C ） , group depression （ group D ） , group propefol （ group DP） , group eleetroconvulsive therapy （ group E） and group propofol combined electroconvulsive therapy （ group M）. The rats of group C were fed normally;the Others were stressed repeatedly for 28 days to establish depression model. The model was evaluated with Open-field and sucrose consumption test. After the model was set up,the rats of group E were gave electroconvulsive therapy once on days for one weeks; the rats of group P were peritoneal injected with propofol （90 mg/kg） ;the rats of group M were gave ECT after anesthetized with propefol （90 mg/kg）, the ethnology was determined by using Open-field method and experiment of sucrose cOnsumption. Brain tissues were dissected to detect TNF-α mRNA expression by RT-PCR and its receptor immunohistochemistry, and the TNF-α content was measured by Enzyme-linked Immunosorbent assayelisa. Results Open-field test and sucrose consumption test：the quantities of rats in group E and group M were higher than that in group D （ P 〈 0.05 ）. and group M was higher than groupE （ P 〈 0.05 ）. The TNF-α contents and TNF-α mRNA expression in hippocampus ： in group D and group DP was higher than that in other groups （ P 〈 0.05 ）. The TNF-α content in hippocampus of rats in group M was lower than that in group E, （ P 〈 0.05 ）, had no significance compared group C （ P 〉 0.05 ）. TNF-α receptor expression in hippocampus CA3 areas by immunohistochemistry：The expression in group C were higher than that of in group DP and group D （P〈0. 05）. and the expression in group M was higher than that in group E （P 〈 0.05 ）. Conclusion The TNF-α expression up-regulated, the TNF-α receptor expression down-regulated then the rats showed depression symptoms Propofol and further down-regulation of TNF-α expression up-regulation of TNF-α receptor expression, so the depression symptoms and t of depressed rats were improved.