腹腔镜手术时不同腹腔环境对大鼠腹腔巨噬细胞的影响(英文)

Effect of various laparoscopic pneumoperitoneum on peritoneal macrophages in rats

目的探讨腹腔镜手术时不同腹腔环境下腹腔免疫功能的改变。方法将32只大鼠雄性SD大鼠随机分为四组:开腹组,无气腹组,C O 2气腹组和N 2气腹组,每组8只。模拟开腹手术和相应腹腔镜手术时的气腹环境,时间为1小时,于48小时后从大鼠的腹腔中分离出巨噬细胞。R T-PC R检测腹腔巨噬细胞分泌iN O Sm R N A、TN F-αm R N A及IL-1βm R N A的表达。结果术后四组相比较,腹腔巨噬细胞数四组间无统计学差异(P>0.05);开腹组腹腔巨噬细胞iN O S m R N A、TN F-αm R N A及IL-1βm R N A的表达最低(P<0.01〉;C O 2气腹组居中(P<0.01);无气腹组和N 2气腹组较高(P<0.01);无气腹组和N 2气腹组间无明显统计学差异(P>0.05)。结论 C O 2气腹对腹腔免疫功能的抑制作用小于开腹组,但大于无气腹组或N 2气腹组。

【Objective】 To investigate the effect of various laparoscopic pneumoperitoneum on peritoneal macrophages in rats.【Methods】 A total of 32 male SD rats were randomly divided into 4 groups with eight rats in each group： laparotomy group,gasless group,CO2 pneumoperitoneum group and N2 pneumoperitoneum group.Laparotomy and corresponding laparoscopic pneumoperitoneum procedures were simulated for 1 h.Macrophages were isolated from the peritoneal cavity of the rats 48 h after the operation,and expressions of iNOS mRNA,TNF-α mRNA and IL-1β mRNA produced by the peritoneal macrophages were measured by RT-PCR.【Results】 Results of the four groups were compared after operation,and there was no significant difference in macrophage numbers between the four groups（P〈 0.05）.The expression of iNOS mRNA,TNF-α mRNA and IL-1β mRNA was the lowest in the laparotomy group（P〈 0.01）,moderate in the CO2 pneumoperitoneum group（P〈 0.01）,and higher in the gasless group and N2 pneumoperitoneum group.But there were no significant differences between the gasless group and N2 pneumoperitoneum group（P〈 0.05）.【Conclusions】 Suppression of peritoneal immune function caused by CO2 pneumoperitoneum laparoscopy is less than that by laparotomy,but greater than that caused by gasless or N2 pneumoperitoneum laparoscopy.