子宫内膜样腺癌中垂体肿瘤转化基因1表达与肿瘤侵袭转移的关系

Expression of PTTG1 in endometrioid carcinoma:relationships with tumor invasion and metastasis.

目的探讨垂体肿瘤转化基因1(PTTG1)调控碱性成纤维细胞生长因子(bFGF)、基质金属蛋白酶-2(MMP-2)蛋白表达在子宫内膜癌发生、侵袭和转移中的作用及意义。方法采用免疫组化方法,结合组织芯片技术,检测2005年1月至2009年9月上海交通大学附属上海市第一人民医院124例子宫内膜样腺癌、28例内膜不典型增生、35例正常内膜组织中PTTG1、bFGF和MMP-2的表达水平,结合临床病理因素进行分析。结果 PTTG1、bFGF和MMP-2在子宫内膜样腺癌中的阳性表达率分别为63.7%、73.4%、50.8%,与正常内膜组和不典型增生组比较,差异有统计学意义(P<0.01)。PTTG1蛋白与手术病理分期显著相关(P<0.01),bFGF表达与肿瘤肌层浸润和淋巴结转移明显相关(P<0.05),而MMP-2蛋白表达与肿瘤淋巴结转移显著相关(P<0.01)。子宫内膜样腺癌组织中PTTG1和bFGF、MMP-2的表达均存在正相关关系(r=0.262,P=0.003;r=0.360,P<0.001)。结论 PTTG1可能上调bFGF和MMP-2蛋白的表达,在子宫内膜癌侵袭和转移中起重要作用。

Objective To investigate whether overexpression of PTFG1, bFGF and MMP-2 is associated with tumorigenesis and progression of endometrioid carcinoma. Methods Tissue microarray and immunohistochemical staining were undertaken in 124 endometrial carcinoma, 28 atypical hyperplasia and 35 normal endometrium samples. Then the relationship between the markers and clinic0pathological features were evaluated. Results The presence of PTTG1, bFGF and MMP-2 protein was significantly increased as lesions progressed from normal endometrium to atypical hyperplasia and car- cinoma, respectively （ P 〈 0. 01 ）. Elevated expression of PTTG1 showed a significantly correlation with FIGO stage （ P 〈 0. 01 ）. In addition, bFGF protein correlated with myometrial invasion and lymph node metastasis （P 〈 0. 05）. Further- more, MMP-2 was markedly related to lymph node metastasis （P 〈 0. 01 ）. A positive relationship was found between PT- TG1 and bFGF expression （r =0. 262, P =0. 003） , there was also a significant positive correlation between PTTG1 and MMP-2 （r = 0. 360, P 〈 0. 001 ）. Conclusion These results indicated that enhanced expression of PTTG1, bFGF and MMP-2 may play a crucial role in carcinogenesis and progression of endometrial carcinoma.