摘要
考察了无溶剂体系中固定化脂肪酶Novozym 435催化小桐籽油制备生物柴油的工艺条件。结果表明,加入硅胶有利于提高反应速率和转化率,且当硅胶存在时,甲醇可一次性加入,简化了实验的操作步骤。当无外加水分存在时,甲醇与小桐籽油的物质的量之比为3,固定化酶用量为小桐籽油质量的7%,硅胶加入量与小桐籽油质量比0.4,在40℃和转速150r/min的条件下,酶催化小桐籽油制备生物柴油反应性能较好,反应24h后,其转化率为89%,连续反应10个批次后,固定化酶活力基本不变。
The preparation conditions of biodiesel from Jatropha oil catalyzed by immobilized lipase （Novozym 435） were investigated in a solvent-free system. The results showed that the silica gel in the system could improve the reaction rate of transesterification and the conversion of the Jatropha oil, and the methanol could be added in one step, which simplified the process. The appropriate conditions for the transesterification were molar ratio of methanol to oil 3, enzyme mass fraction 7% based on oil mass, mass ratio of silica gel to oil 0.4, 40 ℃ and 150 r/min. The conversion of oil with 0.18% of water reached 89% after reaction 24 h. The catalytic performance of the immobilized enzyme was stable and the activity of enzyme decreased little after being reused 10 times.
出处
《化学反应工程与工艺》
CAS
CSCD
北大核心
2011年第4期322-326,共5页
Chemical Reaction Engineering and Technology
基金
广西自然科学基金(0991001)
广西理工科学实验中心重点项目(LGZX201008)
关键词
生物柴油
小桐籽油
固定化酶
无溶剂
biodiesel
Jatropha oil
immobilized lipase
transesterification
