陕西省淡色库蚊抗药性研究

Culex pipiens pallens resistance to eight insecticides in Shaanxi province

目的了解陕西省不同地区淡色库蚊对常用杀虫剂的抗性水平。方法采用区分剂量法测定淡色库蚊幼虫对4种杀虫剂的24 h死亡率;采用半数致死浓度(LC50)法测定淡色库蚊幼虫对8种杀虫剂的LC50。结果区分剂量法测定结果显示,除榆林市淡色库蚊对氯菊酯属初步抗性群体外,其余均为抗性群体;LC50测定结果显示,5个市的淡色库蚊对高效氯氰菊酯、氯菊酯、溴氰菊酯、氯氰菊酯、DDT、DDVP、双硫磷、残杀威的LC50分别为0.001 82～0.012 24、0.008 16～0.094 19、0.000 58～0.005 59、0.002 76～0.020 17、0.100 96～1.296 86、0.144 11～0.512 40、0.001 47～0.007 06、0.234 60～1.182 37 mg/L。宝鸡市淡色库蚊的抗药性普遍较高,除溴氰菊酯、氯氰菊酯、双硫磷3种药剂外,其余抗性倍数均在全省最高,而榆林市淡色库蚊的抗药性则普遍较低,除双硫磷和残杀威外,其余各药抗性倍数均为全省最低。结论蚊虫抗药性的产生与杀虫剂的使用密切相关,在创建卫生城市、卫生县城工作中要科学合理用药,开展抗药性监测,以减缓蚊虫抗药性的产生。

Objective To evaluate the resistance in Culex pipiens pallens to commonly used insecticides in different areas of Shaanxi province.Methods Larval mortality for 4 insecticides and LC50 of 8 insecticides determined via the dosing method and median lethal dose method,respectively.Results The resistance level of Cx.pipiens pallens in all five areas was high except the population in Yulin city,which had elementary resistance to permethrin.The LC50 values for larvae to β-cypermethrin,permethrin,deltamethrin,cypermethrin,DDT,DDVP,parathion and propoxur were 0.001 82-0.012 24,0.008 16-0.094 19,0.000 58-0.005 59,0.002 76-0.020 17,0.100 96-1.296 86,0.144 11-0.512 40,0.001 47-0.007 06,and 0.234 60-1.182 37 mg/L,respectively.The highest resistance level to all pesticides was found in Baoji city,except for deltamethrin,cypermethrin and parathion,while the lowest level was found in Yulin city,except for parathion and propoxur.Conclusion The development of mosquito resistance was closely related with the use of insecticides.Therefore,it is important to ensure appropriate administration and regular resistance surveillance to defer and prevent the occurrence of resistance in mosquitoes.