椭圆叶花锚水溶性多糖的分离纯化及组成分析

Isolation,Purification and Composition Analysis of Water-soluble Polysaccharide from Halenia elliptica

目的:为合理利用椭圆叶花锚提供一定依据,对椭圆叶花锚多糖进行提取分离纯化。方法:椭圆叶花锚经热水浸提,乙醇沉淀得粗多糖。粗多糖经Sevage法和酶法联合脱蛋白,酸性乙醇分级,得初步纯化的多糖HM1、HM2、HM3、HM4。结果:其中HM4经DEAE-Sephadex A-25分离纯化得纯化多糖HM41。HM41经检测为纯多糖。气相色谱分析表明椭圆叶花锚多糖HM1、HM2、HM3、HM4和HM41均为Rha、Ara、Xyl、Man、Gal、Glc六种单糖组成的杂多糖,但单糖的摩尔比不同。结论:多糖HM41为首次从椭圆叶花锚中分离得到的均一多糖。

Objective： To provide scientific cue for the use of Halenia elliptica.Methods： The crude polysaccharide was extracted from Halenia elliptica with hot water and precipitated by ethanol.The crude polysaccharide has been eliminated protein and fractionated by acidic ethanol.Four fractions HM1,HM2,HM3 and HM4 were got respectively. Results： By using DEAE-Sephadex A-25 gel filtration,HM41 was got.GC analysis indicated that HM1,HM2,HM3,HM4 and HM41 were composed of Rha,Ara,Xyl,Man,Gal and Glc.But their molar ratios were different.Conclusion： Polysaccharide HM41 is isolated from Halenia elliptica for the first time.