摘要
目的:探讨应用寿胎丸的不同提取部位对肾虚黄体-抑制(病证结合模型)大鼠流产模型黄体功能的影响。方法:应用羟基脲和米非司酮(RU486)稳定复制SD大鼠肾虚-黄体(病证结合)大鼠流产模型;严格控制实验药物来源,寿胎丸分别按照提取工艺,筛选4种提取方法,分别为寿胎丸水提液(A药液)、醇提液(B药液)、醇提后药渣水提液(C药液)、B+C药液。分别对其进行干预,肉眼观察子宫病变,记录胚胎数,计算流产率;化学发光法检测血清雌二醇(E2)、孕酮(P)的水平。应用实时荧光定量(qRT-PCR)分析大鼠流产模型孕激素受体(PR mRNA)在蜕膜表达的影响。结果:综合各专家对评价指标等值加权,寿胎丸B+C药液能显著降低大鼠的流产率、增加胚胎数;提高血清E2及P内分泌水平,上调PR mRNA表达。结论:通过专家综合评价各指标并赋予权重,进行综合分析寿胎丸B+C药液补肾安胎效果最佳。
Objodive： To explore the Kidney-tonifying and abortion preventing effect of Shou Tai Wan（STW） by different extration methods on the SD Rats′ abortion model.Methods： Applied hydroxycarbamide and mifepristone（RU-486） to establish the abortion model of corpus luteum inhibition due to Kidney deficiency（disease-syndorme combination model） on SD,rats. Treated the model rats with STW formula. Observed the uterus condition and recorded the embryo number and the miscarriage rate of each rat.4 kinds of extractions including water extract of STW（A liquid）,alcohol extract of STW（B liquid）,after the ethanol water extract residue of STW（C liquid） and B＋C liquid.Visual observed the uterine lesions embryos and calculated obortion rate.Used chemluninescence methed to cheek the serum estradiol（E2） and progesterone（P） level.Used quantitative RT-PCR（qRT-PCR） to analyze the different of the PR mRNR between the model group and the treated group.Results： Compared with the model group,the abortion rate of B＋C liquid was greatly deduced and the embryo number of B＋C liquid group,the E2 and P levels were obviously increased in the treated groups.Conclusion： STW（B＋C） has the best effect of tonifying the kidney and preventing abortion.
出处
《中药材》
CAS
CSCD
北大核心
2011年第8期1251-1255,共5页
Journal of Chinese Medicinal Materials
基金
国家自然基金(30772819)
